Ap 1000

Manufactured by Vector Laboratories

Sourced in United States

The AP-1000 is a compact and versatile power supply designed for a wide range of laboratory applications. It features adjustable voltage and current output, as well as built-in safety features to protect both the device and the user. The AP-1000 is a reliable and efficient power solution for various laboratory equipment and experiments.

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Lab products found in correlation

Pvdf membrane, by Merck Group (1 mentions) E021040 01, by EarthOx (1 mentions) Bcip nbt kit, by Promega (1 mentions) Sc 546, by Santa Cruz Biotechnology (1 mentions) Bx41 microscope, by Olympus (1 mentions) Dp80 digital microscope camera, by Olympus (1 mentions) Imagescope software, by Leica (1 mentions) Aperio cs2 slide scanner, by Leica (1 mentions) Cellsens imaging software version 1, by Olympus (1 mentions)

4 protocols using ap 1000

Immunodetection of Pneumococcus and Flu

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For immunohistochemical detection of S. pneumoniae and IAV (H1N1), antigen retrieval was performed with microwave heating (600 W) in 10 mM citric acid (750 ml, pH 6.0) for 12 minutes (min). Lung sections were then incubated with a purified rabbit antibody polyclonal to S. pneumoniae (1:2,000, kindly provided by S. Hammerschmidt) or with a purified goat antibody polyclonal to IAV H1N1 (1:4,000, OBT155, Bio-Rad, Puchheim, Germany) at 4°C overnight. Incubation with an immuno-purified rabbit or goat antibody at the same dilution served as negative controls. Subsequently, slides were incubated with a secondary, alkaline phosphatase-conjugated goat anti-rabbit (1:500, AP-1000, Vector, Burlingame, CA) antibody for 30 min at room temperature. The alkaline chromogen triamino-tritolyl-methanechloride (Neufuchsin) was used as phosphatase substrate for color development. All slides were counterstained with hematoxylin, dehydrated through graded ethanols, cleared in xylene and coverslipped.

Dietert K., Gutbier B., Wienhold S.M., Reppe K., Jiang X., Yao L., Chaput C., Naujoks J., Brack M., Kupke A., Peteranderl C., Becker S., von Lachner C., Baal N., Slevogt H., Hocke A.C., Witzenrath M., Opitz B., Herold S., Hackstein H., Sander L.E., Suttorp N, & Gruber A.D. (2017). Spectrum of pathogen- and model-specific histopathologies in mouse models of acute pneumonia. PLoS ONE, 12(11), e0188251.

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Standardized Western Blot Workflow

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Western blot analysis was carried out using standard methodologies. Alkaline phosphatase conjugated anti-rabbit (AP-1000; RRID:AB_2336194) and anti-mouse (AP-2000; RRID:AB_2336173) IgG (H+L) antibodies from Vector Laboratories (CA, USA) were used as secondary antibodies. The detection was carried out with ECF (GE Healthcare, IL, USA).

Nillegoda N.B., Stank A., Malinverni D., Alberts N., Szlachcic A., Barducci A., De Los Rios P., Wade R.C, & Bukau B. (2017). Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. eLife, 6, e24560.

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BDNF Expression in mPFC and NAc

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Tissue blocks of mPFC and NAc shell were punched 4 h and 1 h after intra-VTA injection respectively. The tissues were homogenized in lysis buffer containing protease and phosphatase inhibitors, followed by centrifugation (13000 rpm, 15 min, 4°C). With the supernatants collected and protein concentrations measured, the supernatants were adjusted to the identical protein concentration across groups. The samples (40 μg protein per lane) were electrophoresed by 10% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). The membranes were thrice rinsed and blocked in 5% w/v skim milk for 2h at 25°C, followed by incubation in primary polyclonal rabbit anti-BDNF (1:1000, sc-546, Santa Cruz, USA,) or polyclonal rabbit anti-β-Tubulin (1:1000, E021040–01, Earthox, USA) antibody overnight at 4°C. Afterwards, the bands were rinsed and incubated in alkaline phosphatase-labeled secondary antibody (1:1000, AP-1000, VECTOR, USA) (2h, 25°C), and were visualized by a BCIP/NBT kit (S3771, Promega, USA). We detected a band of approximately 15 kDa, indicating truncated BDNF. β-Tubulin served as the standard of comparison and the gray scale intensity of bands was analyzed by ImageJ software.

Liu D., Tang Q.Q., Yin C., Song Y., Liu Y., Yang J.X., Liu H., Zhang Y.M., Wu S.Y., Song Y., Juarez B., Ding H.L., Han M.H., Zhang H, & Cao J.L. (2018). BDNF-mediated projection-specific regulation of depressive-like and nociceptive behaviors in mesolimbic reward circuitry. Pain, 159(1), 175.

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Immunohistochemical Detection of Acinetobacter baumannii

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For immunohistochemical detection of A. baumannii, antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (750 mL, pH 6.0) for 12 min. Lung sections were then incubated with a rabbit antibody polyclonal to A. baumannii (1:100, kindly provided by José Ramos-Vivas [33 (link)]) at 4 °C overnight. Incubation with a random rabbit antibody at the same dilution served as negative control. Subsequently, slides were incubated with a secondary, alkaline phosphatase conjugated goat anti-rabbit (1:500, AP-1000, Vector Labs, Burlingame, CA, USA) antibody for 30 min at room temperature. The alkaline chromogen triamino-tritolyl-methanechloride (Neufuchsin) was used as phosphatase substrate for color development. All slides were counterstained with hematoxylin, dehydrated through graded ethanol, cleared in xylene and coverslipped. Slides were analyzed and images taken using a BX41 microscope (Olympus, Hamburg, Germany) with a DP80 Microscope Digital Camera and the cellSens Imaging Software, Version 1.18 (Olympus, Hamburg, Germany). For overviews, slides were automatically digitized using the Aperio CS2 slide scanner (Leica Biosystems Imaging Inc., Vista, CA, USA) followed by image file generation using the Image Scope Software (Leica Biosystems Imaging Inc., Vista, CA, USA) as described [31 (link)].

Wienhold S.M., Brack M.C., Nouailles G., Krishnamoorthy G., Korf I.H., Seitz C., Wienecke S., Dietert K., Gurtner C., Kershaw O., Gruber A.D., Ross A., Ziehr H., Rohde M., Neudecker J., Lienau J., Suttorp N., Hippenstiel S., Hocke A.C., Rohde C, & Witzenrath M. (2021). Preclinical Assessment of Bacteriophage Therapy against Experimental Acinetobacter baumannii Lung Infection. Viruses, 14(1), 33.

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