Total RNA was extracted from cell lines using
Trizol reagent (Invitrogen, CA, USA), and then reverse transcription was performed to form cDNA. After that, so as to test the expressions level of LINC01296 and miR-29c-3p, qRT-PCR was performed using
SYBR Green supermix and
Hairpin-it TM miRNAs qPCR kit (Genepharma, Shanghai, China), based on the manufacturer’s instructions. GAPDH and U6 were used as internal control, and 2
-ΔΔCT method was applied to calculate the data.
The primer sequences were as below:
LINC01296: F: 5′- AAGTGGCACCAGCCTCACT -3′
R: 5′- CGGCCAAGT TCTTTACCATC -3′,
GAPDH: F: 5′- GGAGCGA GATCCCTCCAAAAT -3′
R: 5′- GGCTGTTGTCATACTTCTCATGG-3′
miR-29c-3p F: 5′-GCTGGTTTCATATGGTGG -3′
R: 5′-GAACATGTCTGCGTATCTC -3′
U6: F: 5′-CTCGCTTCGGCAGCACATATACT-3′
R:5′-ACGCTTCACGAATTTGCGTGTC-3′
Xu H., Mao H.L., Zhao X.R., Li Y, & Liu P.S. (2020). MiR-29c-3p, a target miRNA of LINC01296, accelerates tumor malignancy: therapeutic potential of a LINC01296/miR-29c-3p axis in ovarian cancer. Journal of Ovarian Research, 13, 31.