Sybr green supermix

Manufactured by GenePharma

Sourced in China

SYBR Green Supermix is a ready-to-use solution for real-time PCR amplification and detection. It contains SYBR Green I dye, DNA polymerase, dNTPs, and necessary buffers and reagents for efficient and reliable quantification of target DNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

Hairpin it mirnas qpcr kit, by GenePharma (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

SYBR Green

2 protocols using sybr green supermix

Quantifying miR-588 and mRNAs in OVCA Cells

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Total RNAs were extracted from OVCA cell lines using TRIzol reagent (Invitrogen) and then cDNA was obtained by reverse transcription. Subsequently, to detect the levels of miR-588 and mRNAs in A2780 and SKOV-3 cell lines, RT-qPCR was conducted using SYBR Green Supermix and Hairpin-it TM miRNAs qPCR kit (GenePharma) under the instructions of the manufacturer. In this assay, glyceraldehyde-3phosphate dehydrogenase (GAPDH) and U6 acted as internal control for RNA levels, and the data were calculated by the 2^-ΔΔCT method. The primer sequences used in this assay are as follows:
miR-588: forward 5′-GGGUUGGCCACAAUGGGU-3′.
reverse 5′-GTCGTATCCAGTGCGTGT-3′.
U6: forward 5′-CTCGCTTCGGCAGCACA-3′.
reverse 5′-AACGCTTCACGAATTTGCGT-3′.
SRSF6: forward 5′-GGTCGCGACGGCTATGATTA-3′.
reverse 5′-ATCCTTCAAGTCCTGCCTGCCAGC-3′.
GAPDH: forward 5′-CTGGGCTCACTGAGCACC-3′.
reverse 5′-AAGTGGTCGTTGAGGGCAATG-3′.

Su X., Wang B., Zhang B, & Pan S. (2023). MiR-588 acts as an oncogene in ovarian cancer and increases the radioresistance of ovarian cancer cells. Journal of Radiation Research, 64(3), 558-568.

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Quantifying LINC01296 and miR-29c-3p Expression

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Total RNA was extracted from cell lines using Trizol reagent (Invitrogen, CA, USA), and then reverse transcription was performed to form cDNA. After that, so as to test the expressions level of LINC01296 and miR-29c-3p, qRT-PCR was performed using SYBR Green supermix and Hairpin-it TM miRNAs qPCR kit (Genepharma, Shanghai, China), based on the manufacturer’s instructions. GAPDH and U6 were used as internal control, and 2^-ΔΔCT method was applied to calculate the data.
The primer sequences were as below:
LINC01296: F: 5′- AAGTGGCACCAGCCTCACT -3′
R: 5′- CGGCCAAGT TCTTTACCATC -3′,
GAPDH: F: 5′- GGAGCGA GATCCCTCCAAAAT -3′
R: 5′- GGCTGTTGTCATACTTCTCATGG-3′
miR-29c-3p F: 5′-GCTGGTTTCATATGGTGG -3′
R: 5′-GAACATGTCTGCGTATCTC -3′
U6: F: 5′-CTCGCTTCGGCAGCACATATACT-3′
R:5′-ACGCTTCACGAATTTGCGTGTC-3′

Xu H., Mao H.L., Zhao X.R., Li Y, & Liu P.S. (2020). MiR-29c-3p, a target miRNA of LINC01296, accelerates tumor malignancy: therapeutic potential of a LINC01296/miR-29c-3p axis in ovarian cancer. Journal of Ovarian Research, 13, 31.

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