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Sybr green supermix

Manufactured by GenePharma
Sourced in China

SYBR Green Supermix is a ready-to-use solution for real-time PCR amplification and detection. It contains SYBR Green I dye, DNA polymerase, dNTPs, and necessary buffers and reagents for efficient and reliable quantification of target DNA sequences.

Automatically generated - may contain errors

2 protocols using sybr green supermix

1

Quantifying miR-588 and mRNAs in OVCA Cells

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Total RNAs were extracted from OVCA cell lines using TRIzol reagent (Invitrogen) and then cDNA was obtained by reverse transcription. Subsequently, to detect the levels of miR-588 and mRNAs in A2780 and SKOV-3 cell lines, RT-qPCR was conducted using SYBR Green Supermix and Hairpin-it TM miRNAs qPCR kit (GenePharma) under the instructions of the manufacturer. In this assay, glyceraldehyde-3phosphate dehydrogenase (GAPDH) and U6 acted as internal control for RNA levels, and the data were calculated by the 2-ΔΔCT method. The primer sequences used in this assay are as follows:
miR-588: forward 5′-GGGUUGGCCACAAUGGGU-3′.
reverse 5′-GTCGTATCCAGTGCGTGT-3′.
U6: forward 5′-CTCGCTTCGGCAGCACA-3′.
reverse 5′-AACGCTTCACGAATTTGCGT-3′.
SRSF6: forward 5′-GGTCGCGACGGCTATGATTA-3′.
reverse 5′-ATCCTTCAAGTCCTGCCTGCCAGC-3′.
GAPDH: forward 5′-CTGGGCTCACTGAGCACC-3′.
reverse 5′-AAGTGGTCGTTGAGGGCAATG-3′.
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2

Quantifying LINC01296 and miR-29c-3p Expression

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Total RNA was extracted from cell lines using Trizol reagent (Invitrogen, CA, USA), and then reverse transcription was performed to form cDNA. After that, so as to test the expressions level of LINC01296 and miR-29c-3p, qRT-PCR was performed using SYBR Green supermix and Hairpin-it TM miRNAs qPCR kit (Genepharma, Shanghai, China), based on the manufacturer’s instructions. GAPDH and U6 were used as internal control, and 2-ΔΔCT method was applied to calculate the data.
The primer sequences were as below:
LINC01296: F: 5′- AAGTGGCACCAGCCTCACT -3′
R: 5′- CGGCCAAGT TCTTTACCATC -3′,
GAPDH: F: 5′- GGAGCGA GATCCCTCCAAAAT -3′
R: 5′- GGCTGTTGTCATACTTCTCATGG-3′
miR-29c-3p F: 5′-GCTGGTTTCATATGGTGG -3′
R: 5′-GAACATGTCTGCGTATCTC -3′
U6: F: 5′-CTCGCTTCGGCAGCACATATACT-3′
R:5′-ACGCTTCACGAATTTGCGTGTC-3′
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