E. faecalis NBRC 100480 was cultivated and protoplasts were prepared as previously described [23 (link)]. The protoplasts were centrifuged at 7000 rpm for 5 min and resuspended in DMB (5 g/L peptone, 1 g/L yeast extract, 0.1 g/L ferric citrate, 19.45 g/L NaCl, 5.9 g/L MgCl2, 3.24 g/L MgSO4, 1.8 g/L CaCl2, 0.55 g/L KCl, 0.16 g/L NaHCO3, 0.08 g/L KBr, 34 mg/L SrCl2, 22 mg/L H3BO3, 8 mg/L Na2HPO4, 4 mg/L Na2SiO3, 2.4 mg/L NaF, and 1.6 mg/L NH4NO3 [BD, Franklin Lakes, NJ]) containing 300 µg/mL penicillin G. The resulting suspension (5 µL) was diluted with 1 mL of DMB containing 300 µg/mL penicillin G (Wako, Osaka) and incubated at 24 °C.
Nh4no3
Manufactured by BD
NH4NO3 is a chemical compound commonly used as a laboratory reagent. It is the ammonium salt of nitric acid, with the chemical formula NH4NO3. This compound is typically used in various scientific applications and experiments, but its specific functions and uses should be determined by the relevant professionals and experts.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nh4no3
1
Cultivation and protoplast preparation of E. faecalis
2
Cultivating E. faecalis Protoplasts
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E. faecalis NBRC 100480 was cultivated and protoplasts were prepared as previously described (Kami et al., 2019 (link)). The protoplasts were centrifuged at 7000 r.p.m. for 5 min and resuspended in Difco Marine Broth 2216 (DMB; 5 g/L peptone, 1 g/L yeast extract, 0.1 g/L ferric citrate, 19.45 g/L NaCl, 5.9 g/L MgCl2, 3.24 g/L MgSO4, 1.8 g/L CaCl2, 0.55 g/L KCl, 0.16 g/L NaHCO3, 0.08 g/L KBr, 34 mg/L SrCl2, 22 mg/L H3BO3, 8 mg/L Na2HPO4, 4 mg/L Na2SiO3, 2.4 mg/L NaF, and 1.6 mg/L NH4NO3 [BD, Franklin Lakes, NJ]) containing 300 µg/mL penicillin G. 2 mL of DMB containing 300 µg/mL of penicillin G (Wako, Osaka) was added to the resulting suspension (10 µL) and incubated at 24 °C.
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