Cm100 microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CM100 microscope is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of small-scale structures and materials. It provides a core function of magnifying and projecting an image of a specimen onto a fluorescent screen or digital camera for observation and analysis.

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Lab products found in correlation

Ultracut uct, by Leica camera (1 mentions) Tecnai f20xt, by Thermo Fisher Scientific (1 mentions) Vector 22 ftir spectrophotometer, by PIKE Technologies (1 mentions) Zetapals zeta potential analyzer, by Brookhaven Instruments (1 mentions)

3 protocols using cm100 microscope

Ultrastructural Axonal Morphometry

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Tissues for the electron microscopy (EM) were prepared as described previously (Pereira et al., 2010 (link); Zou et al., 2011 (link)). Ultra-thin sections were obtained using Ultracut UCT (Leica) and stained with 2% uranyl acetate and lead citrate. Electron micrographs were taken with a Philips CM100 microscope (FEI). The ratio of axonal diameter/fiber diameter (g-ratio) was acquired using ImageJ software.

Xie Y.J., Zhou L., Wang Y., Jiang N.W., Cao S., Shao C.Y., Wang X.T., Li X.Y., Shen Y, & Zhou L. (2018). Leucine-Rich Glioma Inactivated 1 Promotes Oligodendrocyte Differentiation and Myelination via TSC-mTOR Signaling. Frontiers in Molecular Neuroscience, 11, 231.

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Placental Ultrastructure Analysis

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Samples from normal and GDM placentas were fixed immediately after delivery in 2.5% glutaraldehyde (wt/vol.) in 0.1 mol/l cacodylate buffer for 4 h, post-fixed in 1% osmium and then embedded in Araldite resin. Thin sections were stained with lead citrate and uranyl acetate, and viewed using a Philips CM100 microscope (FEI, Hillsboro, OR, USA). All chemicals were purchased from TAAB Laboratories Equipment (Berks, UK)

Yung H.W., Alnæs-Katjavivi P., Jones C.J., El-Bacha T., Golic M., Staff A.C, & Burton G.J. (2016). Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants. Diabetologia, 59(10), 2240-2250.

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Characterization of Nanodiamonds using TEM

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The morphology and size of the NDs were investigated by transmission electron microscopy (TEM). The TEM samples were prepared by immersing the carbon-coated 200-mesh copper grids into a diluted suspension of NDs (0.25 μg/mL) in deionized water. The grids were subsequently washed with PBS and dried for several hours in a desiccator. The dried grids were analyzed with a Philips CM100 microscope operated at 100 kV. High-resolution TEM was recorded on FEI Tecnai F20XT, 200 kV (FEI, Hilsboro, OR). The hydrodynamic diameter and the zeta potential of the NDs after sonication for 20 min (20 kHz, 2 s on, 1 s off) were measured on a ZetaPALS zeta potential analyzer (Brookhaven Instruments Corporation) by hydrodynamic light scattering and laser Doppler electrophoresis. Finally, the surface chemistry of the NDs was characterized by energy-dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectra (FTIR) using a Bruker Vector-22 FTIR spectrophotometer (PIKE Technologies, USA). For FTIR spectra analysis ND’s powder was mixed in KBr tablet, and spectra were recorded in the range of 400–4000 cm⁻¹ (resolution of 1 cm⁻¹).

Pacelli S., Acosta F., Chakravarti A.R., Samanta S.G., Whitlow J., Modaresi S., Ahmed R.P., Rajasingh J, & Paul A. (2017). Nanodiamond-based injectable hydrogel for sustained growth factor release: Preparation, characterization and in vitro analysis. Acta biomaterialia, 58, 479-491.

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