Ir dye cw800 goat anti mouse

Protein samples were run on Any kD Mini-PROTEAN TGX precast protein gels (BioRad) and transferred to 0.45 μm nitrocellulose membranes (GE Healthcare). Membranes were incubated in the primary antibody of interest overnight and washed three times with TBS-Tween 20. Membranes were then incubated in secondary antibody for 1h and imaged using LI-COR Odyssey FC Imaging System. Primary antibodies used in this study: mouse monoclonal α-FLAG M2 antibody (Sigma-Aldrich, F3165), α-HA high affinity rat monoclonal antibody (Roche; 3F10), α-strep (Genscript A00626), α-phospho-Stat3 (Ser727) (Cell Signaling #9134), α-phospho-Stat3 (Ser754) (Cell Signaling #98543), α-Stat3 (124H6) Mouse mAb (Cell Signaling #9139), α-phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167, α-TRIM14 G-15 (Santa Cruz sc79761), α-TRIM14 (Aviva ARP34737), and α-mouse monoclonal Beta-Actin (Abcam, #6276). Secondary antibodies used in this study: IR Dye CW 680 goat anti-rabbit, IR Dye CW 680 goat anti-rat 680, and IR Dye CW800 goat anti-mouse (LI-COR).

The following primary antibodies were used: rabbit polyclonal hnRNP M (Abcam, #177957), rabbit polyclonal Histone 3 (Abcam, #1791), mouse monoclonal Beta-Actin (Abcam, #6276), mouse monoclonal hnRNP L (Abcam, #6106–100), rabbit polyclonal Beta-Tubulin (Abcam, #179513), mouse monoclonal hnRNP U (Santa-Cruz, sc-32315), DAPI nuclear staining (Thermo Fisher), and mouse monoclonal ANTI-FLAG M2 antibody (Sigma-Aldrich, F3165). Secondary antibodies used were asfollows: IR Dye CW 680 goat anti-rabbit, IR Dye CW800 goat anti-mouse (LI-COR), Alexfluor-488 anti-rabbit and Alexafluo-647 anti-mouse secondary antibodies for immunofluorescence (LI-COR).