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Foxp3 alexa 488

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The FOXP3 Alexa 488 is a laboratory reagent used for the detection and quantification of FOXP3 (Forkhead box P3) protein in biological samples. FOXP3 is a transcription factor that plays a crucial role in the development and function of regulatory T cells. The Alexa 488 fluorophore is conjugated to the FOXP3 antibody, enabling the visualization and analysis of FOXP3-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Tbet pe, by Thermo Fisher Scientific (2 mentions) Facscanto flow cytometer, by BD (2 mentions) Easysep human cd4 t cell enrichment kit, by STEMCELL (1 mentions) Pd1 pe cy7, by BioLegend (1 mentions) Cd27 percp cy5, by BioLegend (1 mentions) Cd10 pe cy7, by BioLegend (1 mentions) Cd69 pe, by BioLegend (1 mentions) Cd127 percp cy5, by BioLegend (1 mentions) Cxcr5 percp cy5, by BioLegend (1 mentions) Cd3 efluor 605nc, by Thermo Fisher Scientific (1 mentions) Cd19 apc cy7, by BioLegend (1 mentions) Cd20 magnetic beads, by Miltenyi Biotec (1 mentions) Cd86 apc, by BioLegend (1 mentions) Icos apc, by BioLegend (1 mentions) Cd4 apc cy7, by BioLegend (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Cd4 apc, by Thermo Fisher Scientific (1 mentions) Cd3 percp, by Thermo Fisher Scientific (1 mentions) Ifn γ efluor450, by Thermo Fisher Scientific (1 mentions) Facsaria 3 sorter, by BD (1 mentions)

Spelling variants of this product

Alexa 488 (1863 citations) FoxP3 Alexa Fluor 488 (7 citations) Anti-Foxp3 Alexa Fluor 488 (3 citations) Anti-FOXP3-Alexa Fluor 488 (clone FJK-16s, (2 citations)

4 protocols using foxp3 alexa 488

1

Comprehensive B and T Cell Phenotyping

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B cells were purified from the patient’s peripheral blood by positive selection using CD20 magnetic beads (Miltenyi Biotec, Cambridge, MA). CD4+ T cells were isolated from the peripheral blood of research subjects using the EasySep human CD4+ T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). The following antibodies were used for flow cytometric staining: CD19 APC-Cy7, CD27 PerCP-Cy5.5, CD10 PE-Cy7, CD21 V450, CD69 PE, CD86 APC, FAS Alexa 647, CD4 APC-Cy7, CD127 PerCP-Cy5.5, CD45RO Alexa 700, CXCR5 PerCP-Cy5.5, PD-1 PE-Cy7, ICOS APC (all from Biolegend, San Diego, CA), CD3 eFluor 605NC (from eBioscience, San Diego, CA), and CD21 BD Horizon V450 (BD). Intracellular staining for FOXP3 Alexa 488 (eBioscience) and T-bet PE was performed using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience) in accordance with the manufacturer’s directions.
Minto H., Mensah K.A., Reynolds P.R., Meffre E., Rubtsova K, & Gelfand E.W. (2019). A Novel ATM Mutation Associated with Elevated Atypical Lymphocyte Populations, Hyper-IgM, and Cutaneous Granulomas. Clinical immunology (Orlando, Fla.), 200, 55-63.
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2

Multicolor Flow Cytometry Immunophenotyping

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Cultured T-cell lines were stained with fluorochrome-conjugated monoclonal antibodies against CD3 (1 μg), gamma-delta TCR, CD4, CD8, CD19, NK1.1 (0.5 μg each) for subset analysis (all antibodies from BD Bioscience, San Diego, CA). Cells were stained with FOXP3 Alexa488 (1 μg), CD3 PerCP (1 μg) and CD4 APC (0.5 μg) for T-regulatory cells (Treg) analysis according to FOXP3 staining protocol (eBioscience, San Diego, CA). Data acquisition was performed on a FACS Canto flow cytometer (BD Biosciences) and was analyzed using the FlowJo software (Tree Star, OR).
Phan-Lai V., Dang Y., Gad E., Childs J, & Disis M.L. (2015). The anti-tumor efficacy of IL-2/IL-21-cultured polyfunctional neu-specific T-cells is TNF-alpha/IL-17 dependent. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(9), 2207-2216.
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3

Multicolor Flow Cytometry Protocol

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For surface and intracellular staining, the monoclonal antibodies listed were used: CD4 (PerCP) (RM4-5); CD192 (CCR2) (Alexa 647) (SA203G11) all from BioLegend (Biozol); CD8 (53-6.7); IL-4 (PE-Cy7) (11B11); IL-5 (PE) (TRFK5); IL-13 (Alexa 488) (eBio13A); IFN-γ (eFluor 450) (XMG1.2); CD154 (PE) (MR1); Foxp3 Alexa 488 (FJK-16s); GATA3 (eFluor 660) (TWAJ); Dead Cell Exclusion Marker (DCE) (efluor 780); DCE (efluor 506); Siglec F (PE) (E50-2440); T-bet (PE) (eBio4B10); IL-13 (eFluor 660) (eBio13A); CD11b (PE) (M1/70); F4/80 (PerCP-Cy5.5) (BM8); Ly-6G (Gr-1) (PE-Cy7) (RB6-8c5); Ly-6C (eFluor 450) (HK1.4); TNF-α (Alexa488) (MP6-XT22) all from eBioscience, San Diego, CA, USA.
For intracellular staining of cytokines and transcription factors cells were fixed and permeabilized using the fix/perm buffer kit (eBioscience, San Diego, CA, USA). FACSCantoII flow cytometer and FACSAriaIII sorter (both BD Bioscience, Heidelberg, Germany) were used for cell analysis. FlowJo software 10.2 was used for final analysis (Tree star Inc., Ashland, OR, USA).
Ahmed N., French T., Rausch S., Kühl A., Hemminger K., Dunay I.R., Steinfelder S, & Hartmann S. (2017). Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response. Frontiers in Cellular and Infection Microbiology, 7, 341.
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4

Multiparametric Flow Cytometry of Immune Cells

Cited 2 times
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Splenocytes and TILs were analyzed by flow cytometry. Lymphocytes from tumor and spleens were isolated as previously described [31 (link),32 (link)]. Flow cytometry was performed as shown previously and anti-mouse CD16/CD32 antibody (BD Pharmingen) was used to block nonspecific binding [33 (link)]. The following fluorochrome-conjugated antibodies were used in 2×106 cells: 0.4 μg CD45 (eBioscience, clone #30-F11), 0.4 μg CD3 (BD Pharmingen, clone #145-2C11), 0.4 μg CD4 (BioLegend, clone #GK1.5), 0.4 μg CD8 (eBioscience, clone #53-6.7), 0.4 μg CD11b (eBioscience clone #M1/70), 0.4 μg GR-1 (BD Pharmingen clone #RB6-8C5), and 1 μg Foxp3-Alexa488 (eBioscience, clone # FJK.16 s). Stained cells were acquired with FACS Canto flow cytometer (BD Bioscience) and 1×106 to 2×106 cells were analyzed with FlowJo software (Tree Star Inc.). Results are reported as total percentage of a cell population or ratio of cell quantities, as indicated.
Liao J.B., Ovenell K.J., Curtis E.E., Cecil D.L., Koehnlein M.R., Rastetter L.R., Gad E.A, & Disis M.L. (2015). Preservation of tumor-host immune interactions with luciferase-tagged imaging in a murine model of ovarian cancer. Journal for Immunotherapy of Cancer, 3, 16.
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