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4 protocols using thioridazine

1

Pharmacological Treatments and Irradiation

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Aripiprazole, quinpirole, and haloperidol were purchased from Tocris (Ellisville, MO, USA). A769662 was obtained from LC Laboratories (Woburn, MA, USA). Thioridazine was obtained from Cayman Chemical (Ann Arbor, MI, USA). Irradiation was performed at room temperature by using a 137Cs gamma‐ray source Gammacell 3000 manufactured by Nordion (Ottawa, ON, Canada).
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Genetically Targeted Immunization and Metabolite Uptake in Mice

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Use of B1–8i+/ and B1–8i+/− Jκ/ genetically targeted BALB/cJ mice was as described35 (link),36 (link). Mice were maintained under specific-pathogen-free conditions and all animal experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee. 6- to 12- week-old mice were immunized i.p. with 50 μg of NP-CGG precipitated in alum to induce GCBCs or NP-Ficoll (Biosearch Technologies) in PBS to induce in vivo activated B cells. Mice were i.p. injected once a day at d9 and d13 post NP-CGG immunization with 22 mg/kg bodyweight Etomoxir (Cayman Chemicals) in 5% DMSO in PBS and 11 mg/kg Thioridazine (Cayman Chemicals) in 0.9% NaCl solution. For in vivo metabolite uptake experiments mice were injected intravenously with 0.171 mg (500 nmol) 2-NBDG (in PBS) or 50 μg BODIPYTM FL C16 (in 2.5% DMSO in PBS) and sacrificed 35 or 60 min later, respectively.
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Genetically Targeted Immunization and Metabolite Uptake in Mice

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Use of B1–8i+/ and B1–8i+/− Jκ/ genetically targeted BALB/cJ mice was as described35 (link),36 (link). Mice were maintained under specific-pathogen-free conditions and all animal experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee. 6- to 12- week-old mice were immunized i.p. with 50 μg of NP-CGG precipitated in alum to induce GCBCs or NP-Ficoll (Biosearch Technologies) in PBS to induce in vivo activated B cells. Mice were i.p. injected once a day at d9 and d13 post NP-CGG immunization with 22 mg/kg bodyweight Etomoxir (Cayman Chemicals) in 5% DMSO in PBS and 11 mg/kg Thioridazine (Cayman Chemicals) in 0.9% NaCl solution. For in vivo metabolite uptake experiments mice were injected intravenously with 0.171 mg (500 nmol) 2-NBDG (in PBS) or 50 μg BODIPYTM FL C16 (in 2.5% DMSO in PBS) and sacrificed 35 or 60 min later, respectively.
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4

Catalog of Diverse Chemical Compounds

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Individual chemicals, i.e., albendazole, alexidine, azelastine, bosutinib, carmofur, cladribine, digoxigenin, dronedarone, emetine, miconazole, pentamidine, pitavastatin, sertraline, solifenacin, sulcanozole, tadalafil, terfenadine, thioridazine, trifluorothymidine, and toremifene were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Additionally, 10-deacetylbaccatin, allyl-thiourea, aminoacridine, bazedoxifene, cephalomannine, cetrimonium bromide, ciclopirox, clomifene, cytarabine, fenbendazole, ouabain, oxethazaine, penfluridol, pyrithione zinc, tioconazole, thioguanine, and vinorelbine were purchased from Sigma-Aldrich. Flubendazole was purchased from LKT Laboratories, Inc. (St. Paul, MN, USA), and gemcitabine and niclosamide were purchased from AdipoGen® Life Science (Liestal, Switzerland), whereas thonzonium bromide and pralatrexate were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
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