Hematoxylin and eosin y solution

STZ, glucose, insulin, and hematoxylin and eosin Y solution were purchased from Sigma‐Aldrich (St. Louis, MO, USA). A C‐peptide ELISA Kit was purchased from BioVision (Eugene, OR, USA). A rat/mouse insulin ELISA Kit was purchased from Merck Millipore (EMD Millipore, Darmstadt, Germany). For immunoblotting, antibodies against β‐actin, p‐AKT, and Bax were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). AKT, p‐AKT, p‐p70S6K, 4EBP1, p‐4EBP1, mTOR, p‐mTOR, Bcl2, insulin, and cleaved caspase 3 were purchased from Cell Signaling Technology (Beverly, MA, USA). A commercial brand of Gryllus bimaculatus powder under the name “D&D (Diabetes & Dietary, Inventor: Dr Lee Sam Goo, South Korea)” was obtained from 239bio Inc. (Ixsan, Chonbuk, South Korea). To create the powder, the growth period of Gryllus bimaculatus was limited to a maximum of 35 days. Crickets were subjected to a 3‐day defecation period, washed three times in distilled water, and then freeze‐dried. The freeze‐dried Gryllus bimaculatus were homogenized, and the powder was stored at −20°C for 4 weeks. Powder manufacturing is based on patents belonging to 239bio Inc., Korea, with the following registration numbers: 10–1686179, 10–1663202, 10–1702851, 10–1716766, 10–1716763, 10–1773851, and 10–1809451).