Triethylamine (P/N 90337, ≥99.5% purity) and 1,1,1,3,3,3‐hexafluoro‐2‐propanol manufactured by Aldrich Chemicals (P/N 105228, ≥99% purity) as well as Triethylamine (P/N 65897, ≥99.5% purity) and 1,1,1,3,3,3‐hexafluoro‐2‐propanol manufactured by Fluka (P/N 42060, ≥99.8% purity) were purchased from Sigma Aldrich (St. Louis, MO, USA). LC‐UV grade solvents (Optima series) and 500 mL low‐density polyethylene bottles were purchased from Fisher Scientific (Pittsburg, PA, USA). LC/MS‐grade solvents (CHROMASOLV series) were purchased from Sigma Aldrich. Polypropylene screw neck vials with cap (12 × 32 mm) were purchased from Waters Corp. (Milford, MA, USA). DNA sequencing grade phosphodiester ssRNA sequences with double thymine overhangs were ordered from Integrated DNA Technology (Coralville, IA, USA) with the sequence 5′‐UCGUCAAGCGAUUACAAGGTT‐3′ (strand 1) and 5′‐TTCCUUGUAAUCGCUUGACGA‐3′ (strand 2). Stock oligonucleotide samples were prepared at a concentration of 100 μmol in their received vials using MS‐grade water. Working samples were prepared at a concentration of 10 pmol/μL in LoBind Eppendorf tubes (Hauppauge, NY, USA) using MS‐grade water prior to transfer to polypropylene sample vials. Mass loads on column were kept constant at 50 pmol or 5 μL injections.
1 1 1 3 3 3 hexafluoro 2 propanol
Manufactured by Merck Group
Sourced in United States
1,1,1,3,3,3-hexafluoro-2-propanol is a fluorinated alcohol compound that is commonly used as a specialty solvent in various laboratory applications. It has a unique set of physical and chemical properties that make it suitable for specific research and analysis purposes. This compound provides a concise and unbiased description of its core function without any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Triethylamine, by Merck Group (2 mentions)
Chloroform, by Scharlab (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Nano ha powder, by Merck Group (1 mentions)
Gel type a, by Merck Group (1 mentions)
Pectin, by Merck Group (1 mentions)
Tin 2 2 ethylhexanoate, by Merck Group (1 mentions)
β butyrolactone, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Metronidazole, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Pds 2, by Johnson & Johnson (1 mentions)
Poly ε caprolactone, by Merck Group (1 mentions)
Agilent 6890 gas chromatograph, by Agilent Technologies (1 mentions)
Agilent chemstation software, by Agilent Technologies (1 mentions)
Trifluoroacetic anhydride, by Merck Group (1 mentions)
0.22 μm syringe filter, by Merck Group (1 mentions)
Recombinant human aβ1 42 peptide, by Merck Group (1 mentions)
Cutsmart buffer, by New England Biolabs (1 mentions)
23 protocols using 1 1 1 3 3 3 hexafluoro 2 propanol
1
Analytical LC-MS Protocol for RNA Oligonucleotides
2
Surfactant-Based Fluorinated Solvent Protocol
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FF and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were bought from Sigma-Aldrich. All the surfactants were sodium dodecyl sulphate, dodecyl sodium sulfonate, and sodium deoxycholate. The cetyl trimethyl ammonium bromide (CTAB), tetradecyl trimethyl ammonicum bromide (TTAB), and cetanecyl trimethyl ammonium chloride (CTAC) were bought from the Aladdin company, with a purity of 95–98%.
3
Polymer Solution Preparation Protocol
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Four different polymers, viz., PAN (Mw: 150,000 g/mol), PLGA (Mw: 60,000 g/mol), PCL (Mw: 80,000 g/mol) and PVA (Mw: 500,000 g/mol) were purchased from Sigma Aldrich (St. Louis, MO, USA). Solvents such as N,N-dimethyl formamide and 1, 1, 1, 3, 3, 3-Hexafluoro-2-propanol (≥99%) were supplied by Sigma Aldrich (St. Louis, MO, USA). All the polymer solutions were prepared separately by adding the required amount of polymer and the solvent into a flask with a magnetic stirring for 24 h.
4
Biomimetic Scaffold Fabrication
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AA, β-glycerophosphate disodium salt hydrate (β-GP), NH, EDC, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), nano-HA powder (HA, diameter less than 200 nm), and Gel (type A) were purchased from Sigma Aldrich Co. (St Louis, MO, USA).
5
Purification and Characterization of PHB
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Pectin (from apple), β-butyrolactone, tin(II) 2-ethylhexanoate,
and 1,1,1,3,3,3-hexafluoro-2-propanol were purchased from Sigma-Aldrich.
Solvents chloroform and n-hexane were of ACS grade
and were purchased from J.T. Baker. All chemicals were used as received.
PHB was purified by dissolving in chloroform followed by filtration
and subsequent precipitation in n-hexane before use.
The absolute Mn and Mw of the purified PHB, characterized by miniDAWN multi-angle
laser light scattering (Wyatt Technology) using dn/dc = 0.144, were 6.0 × 104 and
7.1 × 104 g mol–1, respectively.
The apparent Mn and Mw of the purified PHB, characterized by GPC (SEC RI detector)
(Tosoh, Japan) with polymethylmethacrylate calibration standards,
were 2.5 × 105 and 7.6 × 105 g mol–1, respectively.
and 1,1,1,3,3,3-hexafluoro-2-propanol were purchased from Sigma-Aldrich.
Solvents chloroform and n-hexane were of ACS grade
and were purchased from J.T. Baker. All chemicals were used as received.
PHB was purified by dissolving in chloroform followed by filtration
and subsequent precipitation in n-hexane before use.
The absolute Mn and Mw of the purified PHB, characterized by miniDAWN multi-angle
laser light scattering (Wyatt Technology) using dn/dc = 0.144, were 6.0 × 104 and
7.1 × 104 g mol–1, respectively.
The apparent Mn and Mw of the purified PHB, characterized by GPC (SEC RI detector)
(Tosoh, Japan) with polymethylmethacrylate calibration standards,
were 2.5 × 105 and 7.6 × 105 g mol–1, respectively.
6
Electrospun Antibiotic-Loaded PDS Sutures
7
Gelatin-Collagen Protein Blend Characterization
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The gelatin protein used (Gel) was fish gelatin type B (80‐120 g Bloom), supplied by Henan Boom Gelatin Co. Ltd (China). It presented a protein content of ~98 wt% and was also composed of lipids and moisture with a content of less than 1 wt%. On the other hand, collagen protein (Col) was supplied by Essentia Protein Solutions (Grasten, Denmark). It presented a protein content of ~ 95 wt% and other components, such as lipids and moisture (less than 1 wt%). Poly(ε‐caprolactone) (Mw = 45,000 g/mol) and 1,1,1,3,3,3‐hexafluoro‐2‐propanol (HFIP) were purchased from Sigma Aldrich (Germany).
8
Measurement of KP Metabolites in Biofluids
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To extract KP metabolites, samples were treated with trichloroacetic acid at a final concentration of 5% (w+v) in equal volume and incubated in ice for 5 min, vortexed and then centrifuged (4 °C) for 10 min at 12 000 r.p.m. Supernatant was carefully extracted and filtered with a 0.22 μm syringe filter (Millipore-Merck, Billerica, MA, USA). QUIN and PIC levels in CSF and plasma were examined using gas chromatography–mass spectrometry with the spectrometer operating in electron-capture negative-ionization mode. The method used is described in Smythe et al.33 (link) The internal standards were purchased from Le Research (St Paul, MN, USA). Trifluoroacetic anhydride and 1,1,1,3,3,3-hexafluoro-2-propanol of GC derivatization grade, QUIN, PIC and other organic solvents of analytical-grade were all obtained from Sigma-Aldrich (St Louis, MO, USA). One microliter of sample was injected into an Agilent 6890 gas chromatograph, interfaced to an Agilent 5973 mass selective detector via an auto-sampler Agilent Technologies 7683, and controlled using Agilent ChemStation software (Agilent, Santa Clara, CA, USA). The inter- and intra-assay coefficient of variation was <5%.
9
Synthesis and Characterization of Amentoflavone-like Biflavonoids
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In the present study, we used 8 amentoflavone-like biflavonoids consisting of monoflavonoid dimers linked by C-C covalent bonds (Fig. 1 ). Biflavonoids compounds were prepared as we previously reported (Kang et al., 2005 (link); Sasaki et al., 2010 (link)). Briefly, bilobetin, ginkgetin, isoginkgetin, and sciadopitysin were purified from Gingko biloba (Kang et al., 1990 ; Lee et al., 1995 (link)). Amentoflavone was purified from Selaginella tamariscina (Kang et al., 2005 (link)). Sequoiaflavone, sotetsuflavone, and podocarpuflavone were kindly gifted from Dr. Kiyotaka Koyama at Meiji Pharmaceutical University (Tokyo, Japan) (Sasaki et al., 2010 (link)). The purity of the flavonoids used in the study was greater than 98% (Chang et al., 1993 ). Recombinant human Aβ1–42 peptide purified with 1,1,1,3,3,3-hexafluoro-2-propanol were purchased from Sigma-Aldrich (St. Louis, MO, USA).
10
Poly(A) RNA Quantification Protocol
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1,1,1,3,3,3-Hexafluoro-2-propanol,
acetonitrile, ethanol (LC/MS grade), formic acid, methanol (LC/MS
grade), triethylamine, triethylammonium acetate, phenol:chloroform,
water (LC/MS grade), and isopropanol were all obtained from Sigma-Aldrich
(MO). Custom oligonucleotides were obtained from IDT. (IA) Custom
poly(A) standards were obtained from the Horizon Discovery (CO) DNAPac
RP 4 μm × 2.1 mm × 100 mm HPLC column, 7.5 M LiCl
solution, nuclease-free water, RNase T1, DNase I, PCR strip tubes,
and glass HPLC vials with caps were all obtained from ThermoFisher
Scientific (NJ). RNase H, RNase H buffer, Cutsmart buffer, and HiScribeT7
High Yield RNA Synthesis Kit were obtained from New England Biolabs
(MA). QIAprep Miniprep kit was obtained from QIAgen (MD). CleanCap
Reagent AG and CleanCap Reagent AG (3′ OMe) were purchased
from TriLink Biotechnologies (CA).
acetonitrile, ethanol (LC/MS grade), formic acid, methanol (LC/MS
grade), triethylamine, triethylammonium acetate, phenol:chloroform,
water (LC/MS grade), and isopropanol were all obtained from Sigma-Aldrich
(MO). Custom oligonucleotides were obtained from IDT. (IA) Custom
poly(A) standards were obtained from the Horizon Discovery (CO) DNAPac
RP 4 μm × 2.1 mm × 100 mm HPLC column, 7.5 M LiCl
solution, nuclease-free water, RNase T1, DNase I, PCR strip tubes,
and glass HPLC vials with caps were all obtained from ThermoFisher
Scientific (NJ). RNase H, RNase H buffer, Cutsmart buffer, and HiScribeT7
High Yield RNA Synthesis Kit were obtained from New England Biolabs
(MA). QIAprep Miniprep kit was obtained from QIAgen (MD). CleanCap
Reagent AG and CleanCap Reagent AG (3′ OMe) were purchased
from TriLink Biotechnologies (CA).
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