Lactate dehydrogenase ldh

Male C57BL/6 mice (20–25 g) were obtained from the Laboratory Animal Center of Guangdong Province. One hundred eight mice were included in the study. Febuxostat was purchased from Teijin (Tokyo, Japan), and diluted in 0.5% methylcellulose (Sigma, St. Louis, MO, USA). Dulbecco Modified Eagle’s Medium (DEME), 5-bromo-2-deoxyuridine (BrdU), fetal bovine serum (FBS), 2,3,5-triphenyltetrazolium chloride (TTC) and Evan’s Blue dye were purchased from Sigma-Aldrich (St. Louis, MO, USA). Serum creatine kinase (CK) and lactate dehydrogenase (LDH) commercial kits were obtained from Biovision (Mountain View, CA, USA). Caspase-3 and Caspase-9 activity were measured using commercial kits (Biovision, Mountain View, CA, USA). The Hoechst staining kit was obtained from Promega (Beijing, China). ROS and Mitochondrial Membrane Potential Assay Kits were obtained from Beyotime (Jiangsu, China). Antibodies against Bcl-2 (1:1,000), Bcl-X_L (1:1,000), Bax (1:1,000), Bak (1:1,000), Cytochrome C (1:1,000), Caspase-9 (1:1,000), Caspase-3 (1:1,000), and GAPDH were purchased from Cell Signaling Technology (Beverly, MA, USA). COX IV (1:1,000) was from Bioworld Corporation (Dublin, USA).

Individual blood samples were collected from the aortas of rats in all groups that had been fasted one day before. Serum samples were separated from whole blood by using a blood collection tube (Vacutainer; BD Biosciences, NJ, USA). All blood serum was stored at −80 °C, and anti-Müllerian hormone (AMH) (Elabscience Biotechnology, TX, USA), estrogen (BioVision, Milpitas, MA, USA) and lactate dehydrogenase (LDH) (Abcam, Waltham, MA, USA) activity in serum was analyzed by ELISA kits following the manufacturer’s instructions.