Paeoniflorin was purchased from Abcam (Beverly, MA) and was dissolved in saline; then it is kept at 4°C. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, USA). c-Met inhibitor (SU11274) was purchased from Selleck Chemicals (Houston, TX). Antibodies against c-Met, phospho-c-Met (Y1230/34/35), ROCK1, phospho-limk1(T508), and limk1 were purchased from Abcam (Beverly, MA). Antibodies against GAPDH were purchased from Zhongshangjinqiao Science and Technology Ltd. (Beijing, China). Recombinant human HGF protein was purchased from R&D Systems (Minneapolis, USA). The Upstate® Rho Activation Assay Kit was obtained from EMD Millipore (Lake Placid, USA). Phalloidin was purchased from Abcam (Beverly, MA).
Recombinant human hgf protein
Manufactured by R&D Systems
Sourced in United States
Recombinant human HGF protein is a purified protein produced in HEK293 cells. It is a growth factor that plays a role in cell proliferation, survival, and migration.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Phalloidin, by Abcam (1 mentions)
C met inhibitor su11274, by Selleck Chemicals (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Sto 609, by Bio-Techne (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Lip2000 transfection reagent, by Solarbio (1 mentions)
Puromycin, by Solarbio (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Recombinant human vegf 165 protein, by R&D Systems (1 mentions)
Axitinib, by Selleck Chemicals (1 mentions)
P stat3 9145, by Cell Signaling Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
Bone morphogenetic protein 4 truncated bmp 4, by Fujifilm (1 mentions)
6 protocols using recombinant human hgf protein
1
Paeoniflorin Inhibits c-Met and ROCK1 Signaling
2
HGF and LPS-induced Macrophage Activation
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Raw 264.7 cells (American Type Culture Collection, VA) were grown in DMEM (Welgene, Gyeongsan, Korea) supplemented with 10% FBS (Corning, NY) and antibiotics [100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich, MO)]. Recombinant human HGF protein (R&D systems, MN) and LPS (Sigma Aldrich, MO) were used at appropriate concentrations. STO-609 (CaMKKβ inhibitor, Tocris Bioscience, MO) was used at 5 or 20 μM for experiments.
3
Modulating miR-206 and RTK Signaling in Osteosarcoma
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miR-206 overexpression or inhibition in primary OS cells was achieved by lentiviral transduction. Pseudoviral particles for miR-206 overexpression and miR-206 knockdown (anti-miR-206) and for transduction of miR-negative control (miRNC) were purchased from Fulengen (Guangzhou, China) and were used following manufacturer's instructions. Cells with positive transduction were selected by puromycin (Solarbio, Beijing, China). PAX3 or MET gene overexpression in primary OS cells was achieved by plasmid transfection using Lip2000 transfection reagent (Solarbio). Plasmids for PAX3 or MET gene overexpression were purchased from Solarbio and were used following the manufacturer's instructions. Cells were assayed 48 hours after transfection. Positive transfection was confirmed by verifying the expression of GFP reporter gene encoded in the plasmids.
Recombinant human HGF protein was purchased from R&D system (Shanghai, China) and was used for cell treatment as described by Husmann, et al.21 (link) with modifications. To evaluate Akt1 or Erk1/2 activation, OS cells were serum-deprived for 6 hours before HGF treatment at 100 ng/mL for 30 min. For cell viability, apoptosis, migration, and invasion assay, cells were pretreated with 100 ng/mL of HGF for 16 hours before assay with the presence of HGF at the same concentration.
Recombinant human HGF protein was purchased from R&D system (Shanghai, China) and was used for cell treatment as described by Husmann, et al.21 (link) with modifications. To evaluate Akt1 or Erk1/2 activation, OS cells were serum-deprived for 6 hours before HGF treatment at 100 ng/mL for 30 min. For cell viability, apoptosis, migration, and invasion assay, cells were pretreated with 100 ng/mL of HGF for 16 hours before assay with the presence of HGF at the same concentration.
4
Cell Culture and Reagents for CRC Study
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The human CRC cell lines used in our study were purchased from the American Type Culture Collection (ATCC, USA). HUVEC and CT26 were purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All these cells were cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS), penicillin (107 U/l), and streptomycin (10 mg/l) and incubated at 37 °C and 5% CO2. Recombinant human HGF protein, recombinant human CCL19/MIP-3 beta protein, recombinant human VEGF 165 protein were purchased from R&D systems. Axitinib (VEGF receptor tyrosine kinase inhibitor) and crizotinib (MET inhibitor) were purchased from Selleckchem (Houston, USA) and used in accordance with manufacturer’s instructions.
5
Hepatocellular Carcinoma Cell Culture
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Human HCC cell SMMC‐7721 was purchased from Cell Bank of Xiangya Central Laboratory, Central South University. HepG2 was obtained from the Cell Bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences (Shanghai, China). They were maintained in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37 °C in a humidified atmosphere with 5% CO2. Sorafenib tosylate, regorafenib (BAY 73‐4506), U0126, MK2206 2 HCl and S3I‐201 were purchased from Selleck (Selleck Chemicals, Shanghai, China). Recombinant human HGF protein was purchased from R&D Systems (Minneapolis, MN, USA). Primary antibodies against PARP (9532), Snail (3879), E‐cadherin (3195), vimentin (5741), α‐tubulin (2144), MET (8198), phosphorylated (P)‐MET (3077), ERK (4695), P‐ERK (4370), Akt (2920), P‐Akt (4060), STAT3 (12640) and P‐STAT3 (9145) were purchased from Cell Signaling Technology (Beverly, MA, USA). The primary antibody against GAPDH (AB22131) was obtained from Bioworld Technology, Inc. (St Louis Park, MN, USA).
6
Hepatic Maturation of hiPSC-Derived Endoderm
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hiPSC-derived endoderm was further treated with 1% Penicillin-Streptomycin Solution (×100) (16823191: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 20 ng/mL Recombinant Human HGF Protein (294-HG-005: R&D SYSTEMS, Minneapolis, MN), 20 ng/mL Bone Morphogenetic Protein 4 (truncated) (BMP-4) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 ng/mL bFGF (FGF2) (ReproCELL Inc., Kanagawa, Japan) in RPMI 1640 with L-Gln, liquid medium (30264–85: Nacalai Tesque, Kyoto, Japan), supplemented with 2% KSR (10828028: ThermoFisher Scientific, Tokyo, Japan) for 5 days in initial stage of hepatic maturation.
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