Anti-estrogen-related receptor alpha antibodies were purchased from Millipore (EPR46Y, 1:1,000 dilution) or Cell Signaling Technology (E1G1J, 1:1,000 dilution). Anti-RIPK2 (D10B11, 1:500 dilution), anti-GAPDH antibodies (D16H11, 1:2,500 dilution), anti-mouse IgG-HRP, and anti-rabbit IgG-HRP were purchased from Cell Signaling Technology and used at 1:10,000 dilution. Anti-actin antibody was purchased from Abcam (ab6276, 1:2,000). Anti-Tubulin antibody was purchased from Sigma Aldrich (T9026, 1:5,000 dilution).
E1g1j
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
E1G1J is a laboratory equipment product from Cell Signaling Technology. It is a core component used for a specific function in the research and development process, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse igg hrp, by Cell Signaling Technology (1 mentions)
Anti tubulin antibody, by Merck Group (1 mentions)
T9026, by Merck Group (1 mentions)
Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
Ab6276, by Abcam (1 mentions)
Ab76228, by Abcam (1 mentions)
Ym3029, by Immunoway (1 mentions)
Yt5743, by Immunoway (1 mentions)
Cleaved gsdmd, by Cell Signaling Technology (1 mentions)
Ym3030, by Immunoway (1 mentions)
D3a3z, by Cell Signaling Technology (1 mentions)
D3r6y, by Cell Signaling Technology (1 mentions)
2 protocols using e1g1j
1
Antibody Dilutions for Protein Analysis
2
Comprehensive Immunoblotting Techniques
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Standard techniques were used for protein quantification, separation, transfer, and blotting in KLE and HEC-1A cells. Primary antibodies immunoblotting antibodies were used: ERRα (1: 500, ab76228, Abcam, London, UK), ERRα (1:500, E1G1J, Cell Signaling Technology, Massachusetts, USA), NLRP3 (1:500; 19,771-1AP, Proteintech, Wuhan, China), HIF-1α (1:500; 20,960-1AP, Proteintech, Wuhan, China), cleaved GSDMD (1:500; #36,425, Cell Signaling Technology, Massachusetts, USA), GSDMD (1:500, E9S1X, Cell Signaling Technology, Massachusetts, USA), GSDME (1:500, 84005S, Cell Signaling Technology, Massachusetts, USA), Caspase1 (22,915–1-AP, Proteintech, Wuhan, China), Caspase-1 (YT5743, Immunoway, USA), Caspase-1 (1:500, D7F10, Cell Signaling Technology, Massachusetts, USA), Caspase-3 (1:500, D3R6Y, Cell Signaling Technology, Massachusetts, USA), IL-18 (1:500,10,663–1-AP, Proteintech, Wuhan, China), cleaved-IL-1β (1:500, D3A3Z, Cell Signaling Technology, Massachusetts, USA), β-actin (1:2000, YM3028l, Immunoway, USA), β-tublin (1:2000, YM3030, Immunoway, USA), and GAPDH (1:2000; YM3029, Immunoway, USA).
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