Pcyclinb1

The following antibodies were used for IF and diluted in TBS supplemented with 0.1% Tween20 and 2% BSA: CyclinA2 (1:400; #sc-751; Santa Cruz), CyclinA2 (1:400; #4656; Cell Signaling), PLK1 (1:400; ab14210; Abcam), pTCTP (1:400; #5251; Cell Signaling), pH2AX (1:800; #2577; Cell Signaling), pCyclinB1 (1:400; #ab55184; Abcam), pLaminA/C (1:400; #2026; Cell Signaling), Alexa Fluor 488-Goat anti-Rabbit (1:2000; #A11008 Life Technologies) and Alexa Fluor 555-Goat anti-Mouse (1: 2000; #A21422 Life Technologies). The following antibodies were used for WB and diluted in TBS supplemented with 0.1% Tween20 and 5% milk powder: CDC6 (1:400; #sc-9964 Santa Cruz), CDT1 (1:400; #sc-28262 Santa Cruz), GAPDH (1:20000; #G8795; Sigma), anti-Rabbit HRP (1:2000; #ab6721; Abcam) and anti-Mouse HRP (1:2000; #ab97023; Abcam).

Anti- E-cadherin, Vimentin, Cytokeratin, MMP-9, Caspase-3, Bax, Bcl-2, p-Cdc2, p-Cyclin B1, p-Cyclin D1, p-PI3K, p-AKT, AKT, p-mTOR, mTOR, p-S6, p-AURKA, AURKA, p-GSK3β, β-catenin, N-cadherin and Ki-67 antibodies were purchased from Abcam (Cambridge, MA, USA). Other reagents were purchased from Sigma (St. Louis, MO, USA).