Anti mouse a90 116p

The primary antibody against MINERVA (5122) was purchased from Cell Signaling Technology (Danvers, MA, USA) or from NOVUSBIO (NBP1-88783) for western blot or immunofluorescence; those against β-actin (A300-491A) and HA (A190-108A) were purchased from Bethyl Laboratories (Montgomery, TX, USA); those against FLAG M2 (F1804) and FLAG (F7425) were purchased from Sigma-Aldrich (St. Louis, MO, USA) for western blot; those against FLAG (D6W5B) were purchased from Cell Signaling Technology (Danvers, MA, USA) for immunofluorescence. The secondary antibodies, horseradish peroxidase (HRP)-linked anti-rabbit (A120-101P) and anti-mouse (A90-116P), were purchased from Bethyl Laboratories (Montgomery, TX, USA). Lipofectamine 2000 (11668019) and RNAiMAX (13778150) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Primary antibodies against OGT (24083) and O-GlcNAc (9875) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-actin (A300–491A) were purchased from Bethyl Laboratories (Montgomery, TX, USA), and those against PRKCG (SC-166451) and PDGFRB (SC-374573) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies against horseradish peroxidase-linked anti-rabbit (A120–101P) and anti-mouse (A90–116P) antibodies were purchased from Bethyl Laboratories. For immunohistochemistry (IHC), primary antibodies against OGT (ab96718) and Ki67 (ab15580) were purchased from Abcam (Cambridge, UK), and antibodies against Caspase-3 (9661) were purchased from Cell Signaling Technology. GEM (G6423) was purchased from Selleck Chemicals (Houston, TX, USA). PTX (T7402), Thiamet G (SML0244), MG132 (M7449), and phosphatase inhibitor cocktails 2 and 3 were purchased from Sigma-Aldrich. MTT (M1415) was purchased from Duchefa Biochemie (Haarlem, The Netherlands). Protease-inhibitor cocktail tablets were purchased from Roche Applied Biosciences, and RNAi-Max (13778150) was purchased from Thermo Fisher Scientific.