Assay 360

Venous blood samples after 12 h fasting were collected from each patient on the day before device implantation. All these tests were measured in the core laboratory of Fuwai Hospital by standard techniques. GGT and other biochemical parameters, including creatinine, FBG, HDL, LDL, triglyceride, were determined by Hitachi 7180 biochemistry autoanalyzer. The concentrations of hsCRP were examined using immunoturbidimetry (Beckmann Assay 360, Bera, CA, USA). The Cockcroft-Gault equation was used to estimate glomerular filtration rate.

Blood samples were taken by direct venipuncture from each patient after at least 12-h fasting in the morning. The samples were collected into EDTA-anticoagulant tubes and centrifuged to produce plasma. Plasma NT-proBNP concentration was measured with an electrochemiluminescence immunoassay (ECLIA) method (NT-proBNP, Roche, Germany) by a Roche modular analytics E170 immunoassay analyzer. Fasting blood glucose (FPG) was determined by enzymatic hexokinase method, while glycosylated hemoglobin (HbA1c) was measured using Tosoh Automated Glycohemoglobin Analyzer (HLC-723G8, Tokyo, Japan). Lipid profiles including total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were measured using an automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan) and enzymatic assay. The concentrations of high-sensitivity C-reactive protein (hsCRP) were determined using immunoturbidimetry (Beckmann Assay 360, Bera, CA, USA).

Venous blood samples were obtained from each patient at baseline upon admission. TC and TG were measured by enzymatic methods and HDL-C by a direct method (Roche Diagnostics, Basel, Switzerland). LDL-C was obtained by Friedewald’s formula (if fasting triglycerides < 3.39 mmol/l) or by ultracentrifugation. ApoB was measured by an immunoturbidimetric method (Tina-quant, Roche Diagnostics) calibrated against the World Health Organization/International Federation of Clinical Chemistry reference standard SP3–07. The levels of hemoglobin A1c (HbA1c) were measured using the Tosoh G7 Automate HPLC Analyzer (TOSOH Bioscience, Japan). The TG/HDL-C index was calculated from ratio of fasting TG to HDL-C ratio. Non-HDL cholesterol was calculated by subtracting HDL cholesterol from total cholesterol. LDL-C/HDL-C ratio expressed the ratio of LDL-C and HDL-C. The levels of high-sensitivity-CRP were determined using immunoturbidimetry (Beckmann Assay 360, Bera, Calif., USA) according to our previously reported. The median normal value for hs-CRP is 0.8 mg/L, with 90% of normal values <0.3 mg/L, and a lower detection limit of 0.2 mg/L. The inter-assay and intra-assay coefficients of variation were <5%, respectively. All other included biomarkers were analyzed by standard hematological and biochemical tests.