Rs α cyclopropyl 4 phosphonophenyl glycine cppg

Manufactured by Bio-Techne

Sourced in United Kingdom

CPPG is a selective and potent agonist of group III metabotropic glutamate receptors (mGluRs). It acts as a specific ligand for these receptors, which are involved in the modulation of synaptic transmission and neuronal excitability in the central nervous system.

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Lab products found in correlation

Clozapine, by Merck Group (1 mentions) Ly341495, by Bio-Techne (1 mentions) Mk801, by Fujifilm (1 mentions) S 4 carboxyphenylglycine cpg, by Bio-Techne (1 mentions) Silastic laboratory tubing, by Dow (1 mentions) L 2 amino 4 phosphonobutyric acid l ap4, by Bio-Techne (1 mentions)

Spelling variants of this product

Glycine (11 citations) RS)‐α‐cyclopropyl‐4‐phosphonophenyl glycine (3 citations)

3 protocols using rs α cyclopropyl 4 phosphonophenyl glycine cppg

Pharmacological Modulation of Neurochemical Pathways

Cited 1 time

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Clozapine was purchased from Sigma (St. Louis, MO, USA). The non-competitive NMDA-R antagonist, MK801 [40 (link)], was obtained from Wako Chemicals (Osaka, Japan). The II-mGluR antagonist (LY341495), the III-mGluR antagonist ((RS)-α-cyclopropyl-4-phosphonophenyl glycine (CPPG)) [41 (link)], and the Sxc inhibitor ((S)-4-carboxyphenylglycine (CPG)) [30 (link),42 (link)] were purchased from Tocris Bioscience (Bristol, UK). The hemichannel and gap-junction blocker, carbenoxolone (CBX) [43 (link)], was obtained from Cosmo Bio (Tokyo, Japan).
LY341495 and CBX were initially dissolved in 10 mM with dimethyl sulfoxide, then diluted to 1 mM with modified Ringer’s solution (MRS) or artificial cerebrospinal fluid (ACSF) [28 (link),44 (link)]. CPPG and CPG were dissolved in MRS or ACSF. CLZ and MK801 were dissolved in MRS, ACSF, or Dulbecco’s modified Eagle’s medium containing 10% fetal calf serum (fDMEM) containing less than 0.1% acetic acid.

Fukuyama K., Kato R., Murata M., Shiroyama T, & Okada M. (2019). Clozapine Normalizes a Glutamatergic Transmission Abnormality Induced by an Impaired NMDA Receptor in the Thalamocortical Pathway via the Activation of a Group III Metabotropic Glutamate Receptor. Biomolecules, 9(6), 234.

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Intrathecal Injection of mGluR Agonist and Antagonist in Rats

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The L5 vertebra of rats was laminectomized under adequate anesthesia with sevoflurane (2.5%–5.0% inhalation administration, in air), and a 7-cm soft tube (SILASTIC laboratory tubing, Dow Corning Corporation, Auburn, MI, USA; outer diameter, 0.64 mm) filled with saline was inserted into the subarachnoid space at a length of ∼0.5 cm. After the muscle incision was closed, the tube was laid under the skin and the cut end was ligated. Then, the incision was closed. Ten microliters of group III mGluR agonist L-(+)-2-amino-4-phosphonobutyric acid (L-AP4) (Tocris Bioscience) and group III mGluR antagonist (RS)-α-cyclopropyl-4-phosphonophenylglycine (CPPG) (Tocris Bioscience) were carefully administered, followed by 5 µl of saline. According to previous studies,^{19 (link),26 (link)} the doses of L-AP4 and CPPG were 10 and 50 nmol/µl diluted in phosphate-buffered saline (PBS), respectively. One dose of each drug was given 5 min prior to formalin injection. Rats were anesthetized, and drugs were administered using a Hamilton syringe.

Okubo M., Yamanaka H., Kobayashi K, & Noguchi K. (2019). Differential expression of mGluRs in rat spinal dorsal horns and their modulatory effects on nocifensive behaviors. Molecular Pain, 15, 1744806919875026.

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Synthesis and Characterization of CPPG and Related Compounds

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(RS)-α-cyclopropyl-4-phosphonophenylglycine (CPPG; RRID: SCR_003281) was obtained from Tocris (Minneapolis, MN). L-2-amino-4-phosphonobutyric acid (L-AP4; RRID: SCR_003105), S-3,5-dihydroxyphenylglycine (DHPG, Cat. #D3689), and 2-methyl-6-(phenylethynyl) pyridine (MPEP; Cat. #5046350001) were purchased from Sigma-Aldrich (St. Louis, MO).

Zhou J.J., Pachuau J., Li D.P., Chen S.R, & Pan H.L. (2020). Group III metabotropic glutamate receptors regulate hypothalamic presympathetic neurons through opposing presynaptic and postsynaptic actions in hypertension. Neuropharmacology, 174, 108159.

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