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Las x 3d

Manufactured by Leica
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The LAS-X-3D is a laser scanning confocal microscope system developed by Leica. It is designed to provide high-resolution, three-dimensional imaging capabilities for a variety of scientific and industrial applications.

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Lab products found in correlation

F4 80, by Bio-Rad (2 mentions) Fluoromount g, by Thermo Fisher Scientific (2 mentions) Triton x 100, by Merck Group (2 mentions) Las x software, by Leica (1 mentions) Lipidtox, by Thermo Fisher Scientific (1 mentions) Lysotracker, by Thermo Fisher Scientific (1 mentions) Sp8 laser scanning confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

LAS X 3D Analysis Software v. 2018.7.3 Rendering LAS X 3D Visualization module LAS X 3D Visualization LAS X 3D Version 3.1.0 software LAS X

3 protocols using las x 3d

1

Multimodal Imaging of Omentum Samples

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Human and mouse omentum samples were first incubated with lysotracker (ThermoFisher, 1/1000) for 30 min at 37 °C. Tissues were then fixed for 1 h on ice in 10% NBF (Sigma) prior to staining at room temperature with primary antibodies and LipidTox (1/100, ThermoFisher) for 2 h in PBS 0.5% BSA 0.5% Triton. Antibodies used are listed in Table S1. After mounting with Fluoromount G, confocal images were acquired using a Leica SP8 laser scanning confocal microscope using the Leica LAS X software. Three-dimensional (3D) reconstruction was created using the LAS-X-3D (Leica) v3.5.7.23225 and IMARIS software (2018).
Magalhaes M.S., Smith P., Portman J.R., Jackson-Jones L.H., Bain C.C., Ramachandran P., Michailidou Z., Stimson R.H., Dweck M.R., Denby L., Henderson N.C., Jenkins S.J, & Bénézech C. (2021). Role of Tim4 in the regulation of ABCA1+ adipose tissue macrophages and post-prandial cholesterol levels. Nature Communications, 12, 4434.
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2

Omental Stromal Cell Immunostaining

Cited 1 time
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Omenta were fixed in 2% neutral buffered formalin for 1 h on ice, before staining in the fridge overnight with antibodies to GATA6 (Cell Signalling; clone D61E4; purified) and F4/80 (Serotec; clone CL:A3-1; purified) or TIM4 (Biolegend; clone RMT4-54; conjugated to AF647) in PBS containing 0.5% BSA, 0.5% triton X-100 (Sigma). Omenta were subsequently stained for 1 h at room temperature with indicated antibodies (Table S2) in PBS containing 0.5% BSA, 0.5% triton X-100, with DAPI added for the final 10 min. Samples were mounted with Fluoromount G (ThermoFischer) and confocal images acquired using a Leica SP8 laser scanning confocal microscope using Leica LAS X software. 3D reconstruction was created using LAS-X-3D (Leica) v3.5.7.23225. Gata6 expression in CD45− omental cells was determined by analysis of a previously pubished dataset available under accession number GSM4053741 [23 (link)].
Louwe P.A., Forbes S.J., Bénézech C., Pridans C, & Jenkins S.J. (2022). Cell origin and niche availability dictate the capacity of peritoneal macrophages to colonize the cavity and omentum. Immunology, 166(4), 458-474.
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3

Omental Stromal Cell Immunostaining

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Omenta were fixed in 2% neutral buffered formalin for 1 h on ice, before staining in the fridge overnight with antibodies to GATA6 (Cell Signalling; clone D61E4; purified) and F4/80 (Serotec; clone CL:A3-1; purified) or TIM4 (Biolegend; clone RMT4-54; conjugated to AF647) in PBS containing 0.5% BSA, 0.5% triton X-100 (Sigma). Omenta were subsequently stained for 1 h at room temperature with indicated antibodies (Table S2) in PBS containing 0.5% BSA, 0.5% triton X-100, with DAPI added for the final 10 min. Samples were mounted with Fluoromount G (ThermoFischer) and confocal images acquired using a Leica SP8 laser scanning confocal microscope using Leica LAS X software. 3D reconstruction was created using LAS-X-3D (Leica) v3.5.7.23225. Gata6 expression in CD45− omental cells was determined by analysis of a previously pubished dataset available under accession number GSM4053741 [23 (link)].
Louwe P.A., Forbes S.J., Bénézech C., Pridans C, & Jenkins S.J. (2022). Cell origin and niche availability dictate the capacity of peritoneal macrophages to colonize the cavity and omentum. Immunology, 166(4), 458-474.
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