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Lipofectamine 200 reagent

Manufactured by Thermo Fisher Scientific
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Lipofectamine 2000 reagent is a cationic lipid-based transfection reagent used for the delivery of DNA, RNA, and other macromolecules into mammalian cells. It facilitates efficient and reproducible transfection of a wide variety of cell types.

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Lab products found in correlation

Pgl3 vector, by Promega (1 mentions) Prl tk renilla luciferase reporter vector, by Promega (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Shrna nc, by RiboBio (1 mentions)

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Lipofectamine (2314 citations) Lipofectamine reagent (569 citations) Lipofectamine 200 (30 citations)

4 protocols using lipofectamine 200 reagent

1

Luciferase Assay for miRNA Binding

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The pGL3 vector (Promega, Madison, SUA) was used to construct the reporter vectors, and the fragment of wild‐type 3′UTR of FOXO3 or PERP containing the putative miR‐629 binding site was amplified from human genomic DNA and cloned into the downstream of the pGL3 vector, For the mutant, point mutations were introduced into the seed region of miR‐629 bindings sties. For the luciferase reporter assay, cells were co‐transfected with wild‐type or mutant reporter vectors, respective miRNAs (mimics NC or miR‐629 mimics) and pRL‐TK renilla luciferase reporter vector (Promega) by Lipofectamine 200 reagent (Invitrogen) according to the manufacturer's protocol. Relative luciferase activities were determined by Dual‐Luciferase Reporter Assay system (Promega) at 48 hours after co‐transfections.
Zhao M., Chen N., Li X, & Lin L. (2019). MiR‐629 regulates hypoxic pulmonary vascular remodelling by targeting FOXO3 and PERP. Journal of Cellular and Molecular Medicine, 23(8), 5165-5175.
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2

Targeted Silencing of AMPK and PKC

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Down-regulation of AMPKα1, AMPKα2 and PKCγ was obtained by siRNAs transfection. The siRNAs were purchased from GenePharma. The sequence information was shown in Table S1. Following the manufacturer’s protocol, siRNAs were transfected into tumor cells by using Lipofectamine 200 reagent (Invitrogen). After 48 h transfection, Western blot was applied to evaluate the knockdown efficiency.
Gong Y., Wang C., Jiang Y., Zhang S., Feng S., Fu Y, & Luo Y. (2020). Metformin Inhibits Tumor Metastasis through Suppressing Hsp90α Secretion in an AMPKα1-PKCγ Dependent Manner. Cells, 9(1), 144.
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3

Modulation of SIRT4 in Cardiomyocytes

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Small hairpin RNA (shRNA) specific against SIRT4 (50 nM; shRNA-SIRT4-1, shRNA-SIRT4-2 and shRNA-SIRT4-3) and a non-targeting sequence serving as a negative control (shRNA-NC) were synthesized by Guangzhou RiboBio Co., Ltd. For transfection, primary neonatal rat cardiomyocytes were placed in the wells of six-well plates at 2x105 cells/well and cultured at 37˚C until reaching 70% confluence. Lipofectamine® 200 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was utilized to perform the transfection experiments. At 48 h post-transfection, transfection efficacy was assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Subsequently, transfected cells were treated with H2O2 and/or SFQXD mentioned in the previous section.
Zhang S., Zhang Y., Wang X., Wu L., Shen J., Gu M, & Fang Z. (2021). Effects of Shenfu Qiangxin Drink on H2O2-induced oxidative stress, inflammation and apoptosis in neonatal rat cardiomyocytes and possible underlying mechanisms. Experimental and Therapeutic Medicine, 21(6), 553.
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4

Transfection of Human 5-HT2A Receptor in HEK293 Cells

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Human embryonic kidney (HEK293) cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% (v/v) fetal bovine serum at 37 °C in a 5% CO2-humidified atmosphere. Transfection was performed using Lipofectamine 200 reagent (Invitrogen) according to the manufacturer’s instructions. The N-terminally c-Myc-tagged form of wild type human 5-HT2A receptor (pcDNA3.1-c-Myc-5-HT2A) has been described previously.21 (link)
Younkin J., Gaitonde S.A., Ellaithy A., Vekariya R., Baki L., Moreno J.L., Shah S., Drossopoulos P., Hideshima K.S., Eltit J.M., González-Maeso J., Logothetis D.E., Dukat M, & Glennon R.A. (2016). Reformulating a Pharmacophore for 5-HT2A Serotonin Receptor Antagonists. ACS chemical neuroscience, 7(9), 1292-1299.
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