M6ii 7

Manufactured by Cell Sciences

The M6II-7 is a laboratory equipment product designed for cell culture applications. It functions as an incubator, providing a controlled environment for the growth and maintenance of cell lines. The core function of the M6II-7 is to regulate temperature, humidity, and CO2 levels to support optimal cell culture conditions.

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Lab products found in correlation

Odyssey infrared imager, by LI COR (2 mentions) Alexa fluor 594 goat anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 donkey anti rat, by Thermo Fisher Scientific (2 mentions) Na k atpase, by Abcam (2 mentions) Evos fl auto imaging microscopy, by Thermo Fisher Scientific (1 mentions) A1r confocal microscope, by Nikon (1 mentions)

5 protocols using m6ii 7

Quantifying ABCC6 Protein Levels

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Total proteins from mouse liver were prepared and separated on 8% SDS-PAGE. An anti-ABCC6 antibody M6II-7 (Cell Sciences), 1:500, was used to detect the human ABCC6 protein. A mouse monoclonal anti Na,K-ATPase antibody, 1:500, was used as a plasma membrane marker for equal protein loading. The membrane was incubated with secondary antibodies, 1:20,000, and scanned with an Odyssey Infrared Imager (LI-COR Biosciences, Lincoln, NE). The bands were quantified by densitometry using the LI-COR software.

Kowal L., Huang J., Luo H., Singh J., Snook A.E., Uitto J, & Li Q. (2021). Functional Assessment of Missense Variants in the ABCC6 gene Implicated in Pseudoxanthoma Elasticum, a Heritable Ectopic Mineralization Disorder. The Journal of investigative dermatology, 142(4), 1085-1093.

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Hepatic ABCC6 Protein Localization

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Immunostaining of liver samples was performed on 6 μm frozen sections. The rat monoclonal anti-human ABCC6 antibody M6II-7 (Cell Sciences) was used to identify human ABCC6 protein. A mouse monoclonal antibody was used to label the basolateral plasma membrane marker Na,K-ATPase (Abcam, Cambridge, MA). The Alexa Fluor 488 donkey anti-rat and Alexa Fluor 594 goat anti-mouse secondary antibodies (Invitrogen) were used. Images were acquired with EVOS FL Auto Imaging Microscopy (Thermo Fisher).

Huang J., Snook A.E., Uitto J, & Li Q. (2019). Adenovirus-mediated ABCC6 Gene Therapy for Heritable Ectopic Mineralization Disorders. The Journal of investigative dermatology, 139(6), 1254-1263.

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Adenoviral Transduction of Mouse Liver Cells

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The mouse liver hepatoma cells (ATCC, Manassas, VA) were transduced with recombinant adenoviruses carrying wild-type or mutant ABCC6 cDNAs at different multiplicities of infection (MOI). Human ABCC6 expression were detected 36 hours post transduction by immunostaining using human ABCC6-specific antibody M6II-7 (Cell Sciences, Newburyport, MA), as previously described (Huang et al., 2019 (link)). The transduction efficiency was quantified by the percentage of positively stained cells.

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Mouse Liver Plasma Membrane Protein Detection

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Plasma membrane proteins from mouse liver were prepared and separated on 8% SDS-PAGE. An anti-ABCC6 antibody M6II-7 (Cell Sciences) was used to detect the human ABCC6 protein. A mouse monoclonal anti Na,K-ATPase antibody was used as a plasma membrane marker for equal protein loading. To visualize the signal, the membrane was incubated with secondary antibodies (LI-COR, Lincoln, NE) and scanned with an Odyssey Infrared Imager (LI-COR).

Huang J., Snook A.E., Uitto J, & Li Q. (2019). Adenovirus-mediated ABCC6 Gene Therapy for Heritable Ectopic Mineralization Disorders. The Journal of investigative dermatology, 139(6), 1254-1263.

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Immunostaining of Liver ABCC6 and Na,K-ATPase

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Immunostaining of liver was performed on 6 μm frozen sections. The rat monoclonal anti-human ABCC6 antibody M6II-7 (Cell Sciences), 1:100 dilution, was used to identify the human ABCC6 protein. A mouse monoclonal antibody, 1:100 dilution, was used to label the basolateral plasma membrane marker Na,K-ATPase (Abcam, Cambridge, MA). The Alexa Fluor 488 donkey anti-rat and Alexa Fluor 594 goat anti-mouse secondary antibodies (Life Technologies), both at 1:400 dilution, were used. Primary and secondary antibodies were incubated at 4°C overnight and at room temperature for 1 hour, respectively. Images were acquired using A1R+ Nikon confocal microscope (Nikon Instruments Inc., Melville, NY).

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