6 aza

Manufactured by Merck Group

Sourced in United States

6-AZA is a chemical compound primarily used as a laboratory reagent. It functions as a nucleoside analog, which can be utilized in various research applications involving nucleic acid analysis and manipulation. The core function of 6-AZA is to serve as a tool for studying and experimenting with biological systems and processes.

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Lab products found in correlation

Biomol, by Enzo Life Sciences (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi medium, by Welgene (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Welgene (1 mentions) Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)

Spelling variants of this product

6-aza-2-thiothymine (5 citations) 8-aza-2,6-dia-minopurine (8-ADP) (2 citations)

2 protocols using 6 aza

High-Throughput Screening of Compound Libraries

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High-throughput screening of compound libraries was conducted at the Stanford University High-Throughput Bioscience Center (HTBC). The libraries which were provided by HTBC consisted of ChemDiv (50K), SPECS (30K), Chembridge (23.5K), ChemDiv Kinase (10K), Library of Pharmacologically Active Compounds (LOPAC1280), NIH Clinical Collection (NIHCC) (446 compounds), NCI DTP (3084 compounds), Microsource Spectrum (2000 compounds), Biomol (now Enzo Life Sciences) ICCB Known Bioactives (480 compounds), and FDA Approved Drug Library (640 compounds). M2-8 or G5 cells were used in screens and were seeded in 384-well white plates at a density of 3000 cells/well. The compound concentration for screening was 10 µM.
The compounds 6-AZA and 6-AZA-TA were purchased from Sigma-Aldrich.

Deng N., Wu Y.Y., Feng Y., Hsieh W.C., Song J.S., Lin Y.S., Tseng Y.H., Liao W.J., Chu Y.F., Liu Y.C., Chang E.C., Liu C.R., Sheu S.Y., Su M.T., Kuo H.C., Cohen S.N, & Cheng T.H. (2022). Chemical interference with DSIF complex formation lowers synthesis of mutant huntingtin gene products and curtails mutant phenotypes. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2204779119.

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Evaluating Anticancer Drug Cytotoxicity

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H460, H1299, MCF-7, HCT116, MIA PaCa-2, H2108, H2199, A549, HeLa, Caco-2, and BxPC-3 cells were cultured in DMEM (Welgene, Korea) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA). H2052 cells were grown in RPMI medium (Welgene) with 10% fetal bovine serum. Cell proliferation was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Briefly, cells were seeded in a 24-well plate, incubated overnight, and treated with the antitumor drugs. At the indicated time, MTT solution was added to a final concentration of 1 mg/mL, and the mixture was incubated for an additional 3 h. MTT was purchased from USB Corporation (Cleveland, OH, USA). 6-AZA, bafilomycin A1, and CQ were purchased from Sigma Aldrich (St. Louis, MO, USA). Natural product library was obtained from the Korea Bioactive Natural Material Bank (KBNMB).

Cha Y.E., Park R., Jang M., Park Y.I., Yamamoto A., Oh W.K., Lee E.J, & Park J. (2021). 6-Azauridine Induces Autophagy-Mediated Cell Death via a p53- and AMPK-Dependent Pathway. International Journal of Molecular Sciences, 22(6), 2947.

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