Hrp conjugated anti rabbit igg

We performed Western blotting according to the protocol described in a previous study [51 (link)]. The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) for Western blotting: anti-cleaved Caspase3 (Cat#9661), anti-cleaved Caspase9 (Cat#9509), anti-cleaved PARP-1 (Cat#9541), anti-JAK1 (Cat# 3344), anti-p-JAK1 (Cat#74129), anti-STAT3 (Cat#9139), anti-p-STAT3 (Cat#9145), anti-p-PI3K (Cat#4228), anti-p-RAF1 (Cat#9427), anti-p-ERK (Cat#4370), anti-AKT (Cat#4691), and anti-p-AKT (Cat#4060). The anti-14-3-3-θ (Cat# ab183075) antibody was purchased from Abcam (Cambridge, UK). The anti-GAPDH (Cat#AC002) antibody was purchased from ABclonal Technology (Wuhan, China). HRP-conjugated anti-rabbit IgG (Cat#AS014) and HRP-conjugated anti-mouse IgG (Cat#AS003) (ABclonal Technology, Wuhan, China) were used as secondary antibodies, and enhanced chemiluminescence reagent (Vazyme, Nanjing, China) was used for detection of signals after exposure of the membrane in an image analyzer (Bio-Tanon, Shanghai, China).