Animals used in this study were 8-week-old male Wistar rats weighing 318 ± 30 g (mean ± SD), which had been purchased from Jackson Laboratory Japan, Yokohama, Japan. The rats were maintained under a 12-h light and 12-h dark cycle in a specific pathogen-free condition with standard chow and water given ad libitum during the entire experiment period, including accommodation to new environment, in the Divisions of Bioresources, Frontier Science Research Center, University of Miyazaki. L-noradrenaline bitartrate monohydrate, azelnidipine, and hydralazine were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The present study was performed in accordance with the Animal Welfare Act and with approval of the University of Miyazaki Institutional Animal Care and Use Committee (No. 2017-509 and No. 2023-502).
L noradrenaline bitartrate monohydrate
Manufactured by Tokyo Chemical Industry
Sourced in Japan
L-noradrenaline bitartrate monohydrate is a chemical compound used in laboratory applications. It is the bitartrate salt of the neurotransmitter L-noradrenaline, also known as L-norepinephrine. The monohydrate form indicates the presence of one water molecule in the crystal structure.
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Lab products found in correlation
Isoproterenol hydrochloride, by Merck Group (2 mentions)
U0126, by Fujifilm (2 mentions)
Phenylephrine hydrochloride, by Fujifilm (2 mentions)
Atipamezole hydrochloride, by Merck Group (2 mentions)
Dexmedetomidine hydrochloride, by Merck Group (2 mentions)
Sapk jnk, by Cell Signaling Technology (2 mentions)
Phospho sapk jnk, by Cell Signaling Technology (2 mentions)
Phospho p38, by Cell Signaling Technology (2 mentions)
Forskolin, by Fujifilm (2 mentions)
Propranolol hydrochloride, by Fujifilm (2 mentions)
Prazosin hydrochloride, by Tokyo Chemical Industry (2 mentions)
Sp600125, by Fujifilm (2 mentions)
Phospho erk1 2, by Cell Signaling Technology (2 mentions)
Wistar rats, by Jackson ImmunoResearch (1 mentions)
Bay 11 7082, by Cayman Chemical (1 mentions)
1 oleoyl lysophosphatidic acid lpa, by Cayman Chemical (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Phospho stat3, by Cell Signaling Technology (1 mentions)
3 protocols using l noradrenaline bitartrate monohydrate
1
Wistar Rat Hypertension Model
2
MAPK Signaling Pathway Antibodies
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Antibodies against ERK1/2 (#4695S, 1:2500), phospho-ERK 1/2 (#9101S, 1:2500), p38 (#9212S, 1:2000), phospho-p38 (#9211S, 1:1000), SAPK/JNK (#9252S, 1:2500), phospho-SAPK/JNK (#9251S, 1:1500), and p65 (#8242S, 1:100) were purchased from Cell Signaling Technology (Danvers, MA, USA). Dexmedetomidine hydrochloride, atipamezole hydrochloride, isoproterenol hydrochloride, and lipopolysaccharides (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phenylephrine hydrochloride, propranolol hydrochloride, forskolin, U0126, and SP600125 were purchased from Wako Pure Chemical (Osaka, Japan). L-noradrenaline bitartrate monohydrate and prazosin hydrochloride were purchased from Tokyo Chemical Industry (Tokyo, Japan). BAY-11-7082 and 1-oleoyl lysophosphatidic acid (LPA) were purchased from Cayman Chemical (Ann Arbor, MI, USA).
3
Molecular Signaling Pathway Analysis
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Antibodies against ERK1/2 (#4695S, 1:2500), phospho-ERK 1/2 (#9101S, 1:2500), p38 (#9212S, 1:2000), phospho-p38 (#9211S, 1:1000), SAPK/JNK (#9252S, 1:2500), phospho-SAPK/JNK (#9251S, 1:1500), STAT3 (#4904S, 1:4000), and phospho-STAT3 (#9145S, 1:2000) were purchased from Cell Signaling Technology (Danvers, MA, USA).
Antibody against CREB (#sc-377154, 1:500) and phospho-CREB (#sc-81486, 1:250) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A peroxidaseconjugated mouse anti-glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; #G9295, 1:50000), dexmedetomidine hydrochloride, atipamezole hydrochloride, and isoproterenol hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Phenylephrine hydrochloride, propranolol hydrochloride, forskolin, U0126, and SP600125 were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). L-noradrenaline bitartrate monohydrate and prazosin hydrochloride were purchased from Tokyo Chemical Industry (Tokyo, Japan). H89 and bisindolylmaleimide II (BIM) were purchased from Cayman Chemical (Ann Arbor, MI, USA).
Antibody against CREB (#sc-377154, 1:500) and phospho-CREB (#sc-81486, 1:250) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A peroxidaseconjugated mouse anti-glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; #G9295, 1:50000), dexmedetomidine hydrochloride, atipamezole hydrochloride, and isoproterenol hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Phenylephrine hydrochloride, propranolol hydrochloride, forskolin, U0126, and SP600125 were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). L-noradrenaline bitartrate monohydrate and prazosin hydrochloride were purchased from Tokyo Chemical Industry (Tokyo, Japan). H89 and bisindolylmaleimide II (BIM) were purchased from Cayman Chemical (Ann Arbor, MI, USA).
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