Fitc conjugated cd24
FITC-conjugated CD24 is a laboratory reagent used to detect the presence and expression levels of the CD24 cell surface antigen. CD24 is a glycosylphosphatidylinositol-anchored sialoglycoprotein that is expressed on a variety of cell types. The FITC (fluorescein isothiocyanate) conjugate allows for the fluorescent labeling of CD24-positive cells, enabling their identification and analysis using flow cytometry or other fluorescence-based techniques.
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5 protocols using fitc conjugated cd24
CD44 and CD24 Expression Analysis
Lineage Tracing of Mammary Tumor Cells
Phenotypic Characterization of Colon Cancer Cells
Flow Cytometry Analysis of NPC Markers
For ECM analysis, cells were fixed and permeabilized with IntraPrep Permeabilization Reagent (A07803, Beckman Coulter). Cells were then subjected to three cycles of freezing (−80 °C) and thawing (37 °C). Subsequently, cells were incubated overnight at 4 °C with primary antibodies against collagen II (NB600-844, Novus Biologicals, CO, USA) and proteoglycan (MAB2015, Sigma-Aldrich). After washing with PBS, cells were reacted with goat anti-mouse Alexa Flour 488-conjugated secondary antibody (A28175, Invitrogen) on ice for 1 h in the dark, and then analyzed by flow cytometry.
Pancreatic Cancer Cell Immunophenotyping
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