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2 protocols using fitc conjugated anti mouse 89750

1

Apoptosis and Autophagy Regulation Study

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The bafilomycin A1 (B1793), 3MA (M9281) and sodium selenite (S-5261) were obtained from Sigma (St. Louis, MO, USA). The antibodies used for western blotting, including anti-IKK alpha (#2682S), anti-Beclin-1 (#3496P), anti-LC3B (#3868P), anti-caspase 3 (#9661S) and anti-PARP (#9546S), were obtained from Cell Signaling Technology (Danvers, MA, USA), whereas anti-P73 antibodies were purchased from Abgent (San Diego, CA, USA), and the anti-UVRAG (sc-82115) and anti-B23 (sc-32256) antibodies for western blots were purchased from Santa Cruz (Santa Cruz, CA, USA). For indirect immunofluorescence, anti-IKK alpha (ab105923) antibodies were obtained from Abcam (Cambridge, UK), whereas anti-LC3 antibodies (M152-3) were purchased from MBL (Woburn, MA, USA) and anti-P73 antibodies (bs-1346R) were purchased from BIOSS (Beijing, China). For immunohistochemical staining, anti-IKK alpha (sc-7183) antibodies were purchased from Santa Cruz. Anti-survivin-2B (ab3729) was purchased from Abcam. The HRP-conjugated anti-goat (ZB-2306), anti-mouse (ZB-2305) and anti-rabbit (ZB-2301) antibodies were obtained from ZSGB-BIO (Beijing, China). The Cy3-conjugated anti-rabbit (89856) and FITC-conjugated anti-mouse (89750) antibodies were purchased from Jackson (West Grove, PA, USA).
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2

Recombinant Proteins and Antibodies for p53/p70S6K Signaling

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Active p70S6K recombinant protein, anti-β-actin antibody, bafilomycin and sodium selenite were purchased from Sigma-Aldrich (St Louis, MO, USA). Pifithrin-α and MnTMPyP were purchased from Merck Calbiochem (San Diego, CA, USA). Anti-p53 antibody and MnTBAP was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-ULK1 and anti-LC3 antibodies (for immunofluorescence) were purchased from Abgent (San Diego, CA, USA). Anti-p-p53 (Ser392) antibody was purchased from Nanjing EnoGene Biotechnology (Nanjing, China). Anti-p70S6K antibody was obtained from Proteintech Group, Inc. (Chicago, IL, USA). The HRP-conjugated anti-mouse (ZB-2305) and anti-rabbit (ZB-2301) antibodies were obtained from ZSGB-BIO (Beijing, China). Anti-p-p70S6K, anti-LC3 and DyLight 488-conjugated anti-rabbit secondary antibody were purchased from Cell Signalling Technology (Danvers, MA, USA). The Cy3-conjugated anti-rabbit (89856) and FITC-conjugated anti-mouse (89750) antibodies were purchased from Jackson ImmunoResearch (West Grove, PA, USA). p53 recombinant protein was obtained from Boston Biochem (Cambridge, MA, USA).
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