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Dfbp40

Manufactured by R&D Systems
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The DFBP40 is a laboratory equipment product manufactured by R&D Systems. It is designed to perform a specific function, but a detailed and unbiased description cannot be provided without the risk of extrapolation or interpretation.

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Lab products found in correlation

Drp300, by R&D Systems (1 mentions) Drsn00, by R&D Systems (1 mentions) Hyaluronidase h3506, by Merck Group (1 mentions)

4 protocols using dfbp40

1

Plasma Adipokine Levels Assessment

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Plasma adipokine levels were analyzed twice for each sample using commercial enzyme-linked immunosorbent assays (ELISA) as described previously [12 (link), 13 (link)]. The adiponectin ELISA assay (catalogue: DRP300, R&D Systems) detection limit was 0.891 ng/ml, and the assay range was 3.9-250 pg/ml. The resistin assay (catalogue: DRSN00, R&D Systems) detection limit was 0.055 ng/ml, and the assay range was 0.2-10 ng/ml. The FABP4 assay (catalogue: DFBP40, R&D Systems) detection limit was 14.2 ng/ml, and the assay range was 62.5-4,000 ng/ml. The visfatin assay (catalogue: K4907, BioVision, Inc., Minneapolis, MN) detection limit was 1.65 pg/ml, and the assay range was 6-400 pg/ml. All ELISA experiments were conducted according to the manufacturer's instructions as described previously [14 (link)–16 (link)].
Chen Y.Z., Zhou S.Q., Chen Y.Q., Peng H, & Zhuang Y.G. (2020). Plasma Adipokines in Patients Resuscitated from Cardiac Arrest: Difference of Visfatin between Survivors and Nonsurvivors. Disease Markers, 2020, 9608276.
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2

Biomarker Levels in AML Patients

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To estimate the status of adipogenesis and osteogenesis in samples derived from AML patients, secreted levels of the biomarkers human fatty-acid-binding protein 4 (FABP4; R&D Systems, cat. #DFBP40) and human osteocalcin (R&D Systems, cat. #DSTCNO) were measured in originally collected, frozen and thawed BM plasma (BMP) samples, obtained from HDs and AML patients at diagnosis. The sensitivity of the ELISA kits was 38 pg/mL for FABP4 and 0.898 ng/mL for osteocalcin.
Sabbah R., Saadi S., Shahar-Gabay T., Gerassy S., Yehudai-Resheff S, & Zuckerman T. (2023). Abnormal adipogenic signaling in the bone marrow mesenchymal stem cells contributes to supportive microenvironment for leukemia development. Cell Communication and Signaling : CCS, 21, 277.
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3

Adipogenic FABP4 Protein Quantification

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Protein was collected from primary fibroblasts following 14 days of incubation in the adipogenic cocktail as described above. Briefly, 50 µL of RIPA buffer (ThermoFisher Scientific, catalogue # 89900) was added to each well for 5 min. Cells were collected after scraping with a cell scraper and immediately frozen at −80°C until analysis for FABP4 protein via ELISA (R&D Systems, catalogue # DFBP40). A Bradford protein assay was used to assess protein concentration of each sample.
Reynolds L.J., Dickens B.J., Green B.B., Marsit C.J, & Pearson K.J. (2016). Using Neonatal Skin to Study the Developmental Programming of Aging. Experimental gerontology, 94, 93-98.
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4

Quantification of FABP4 in Plasma, SF, and Adipose Tissues

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In the first study, plasma FABP4 was determined with an ELISA commercial kit (DFBP40, RandD Systems, MN, USA) according to the manual book provided. Intra-assay and interassay coefficients of variation were 4.6 and 12.1%, respectively. Plasma underwent 20fold dilution before analysis. In the second study, the FABP4 concentration of plasma, SF and culture medium of ScAT and IPFP was determined with another FABP4 quantitative ELISA kit made by our own institution (31030, Antibody and Immunoassay Services, HKU). Intra-assay and interassay coefficients of variation were less than 4.1 and4.5%, respectively. Specifically, SF after thawed was pretreated with 2mg/ml hyaluronidase (H3506, Sigma) at room temperature for 1h on a shaker. Plasma, SF and culture medium of ScAT and IPFP underwent threefold dilution, tenfold dilution and 400-fold dilution before analysis, respectively. The FABP4 concentration from adipose tissues was adjusted by the weight of seeded tissue, and the mean value of three wells from same tissue was calculated. The coefficient of variation (CV) was calculated, defined as the ratio of standard deviation (SD) divided by mean of the values of three wells. If CV>15%, data were considered as largely variable and therefore were not included in the statistical analysis.
Zhang C., Zhang C., Li T., Chiu K.Y., Wen C., Xu A, & Yan C.H. (2018). FABP4 as a biomarker for knee osteoarthritis. Biomarkers in medicine, 12(2).
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