JQ1 was purchased from Tocris Bioscience (Bristol, UK). The recombinant human TGF-β1 was from PeproTech (Rocky Hill, NJ, USA). Antibodies used in Western blot and sources were as follows. The rabbit anti-Brd4 antibody was supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-α-SMA, anti-fibronectin and anti-collagen IV antibodies were purchased from Abcam (Cambridge, UK). Rabbit anti-Smad3, anti-p-Smad3, anti-ERK1/2, anti-p-ERK1/2 and anti-Nox4 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The rabbit anti-GAPDH antibody was from Bioss (Beijing, China). DCFH-DA was from Beyotime Biotechnology (Jiangsu, China), Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit from Molecular Probes (Eugene, OR, USA), polyethylene glycol (PEG)–catalase and N-acetyl-cysteine (NAC) from Sigma-Aldrich (St. Louis, MO, USA), SIS3 from Cayman Chemical (Ann Arbor, MI, USA), and U0126 from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Rabbit anti gapdh antibody
Manufactured by Bioss Antibodies
Sourced in China
Rabbit anti-GAPDH antibody is a primary antibody that specifically recognizes and binds to the GAPDH protein, a widely expressed enzyme involved in glycolysis. This antibody can be used for the detection and quantification of GAPDH in various biological samples, such as cell lysates, tissues, and Western blot analysis.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human tgf β1, by Thermo Fisher Scientific (1 mentions)
Amplex red hydrogen peroxide peroxidase assay kit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti smad3, by Cell Signaling Technology (1 mentions)
Anti collagen 4, by Abcam (1 mentions)
Anti fibronectin, by Abcam (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
U0126, by Santa Cruz Biotechnology (1 mentions)
Anti nox4, by Cell Signaling Technology (1 mentions)
Rabbit anti α sma, by Abcam (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
Anti p smad3, by Cell Signaling Technology (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Affinipure goat anti rabbit igg, by ZSGB-BIO (1 mentions)
0.45 mm polyvinylidene fluoride membranes, by Merck Group (1 mentions)
Pierce ecl plus western blotting substrate kit, by Thermo Fisher Scientific (1 mentions)
Immun blot pvdf membranes, by Solarbio (1 mentions)
Radioimmunoprecipitation assay buffer, by Solarbio (1 mentions)
3 protocols using rabbit anti gapdh antibody
1
Molecular Pathways in Fibrosis Regulation
2
Western Blot Analysis of ILF2 in Gastric Cancer
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Total proteins were extracted from 21 paired fresh frozen GC specimens and corresponding adjacent normal gastric specimens using RIPA lysis buffer and PMSF (Beyotime, China). The protein concentration was detected by BCA protein assay kit (Beyotime, China). Subsequently, an equivalent amount of protein of each paired specimen was separated by SDS-PAGE on 10% polyacrylamide gels and then was electrotransferred to 0.45 mm polyvinylidene fluoride membranes (Millipore, USA) for 1 h at 200 ma. After blocking in 5% nonfat milk diluted with TBST (tris-buffered saline/Tween-20) for 1 h at room temperature, the membranes were separately incubated with rabbit anti-ILF2 monoclonal antibody (1 : 3000; Abcam) and rabbit anti-GAPDH antibody (1 : 3000; Bioss) at 4°C overnight. On the second day, after washing 3 times with TBST per 10 min, the membranes were incubated with peroxidase-conjugated AffiniPure goat anti-rabbit IgG (1 : 6000; zsgb) for 1 h at room temperature. Finally, after washing 3 times with TBST per 10 min, the targeted protein was detected with the enhanced chemiluminescence system according to the manufacture's instruction. The intensity of ILF2 protein band was quantified by ImageJ software and normalized with GAPDH.
3
Sperm Protein Expression Analysis
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Sperm samples were lysed at 4°C in radioimmunoprecipitation assay buffer (Solarbio, R0010) mixed with protease and phosphatase inhibitors. Equal amounts of protein were boiled for 10 min and centrifuged at 16,000 RPM for 5 min. The supernatant was isolated and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE ) loading buffer (P1015, Solarbio, China) was added and subjected to SDS-PAGE. Proteins were then transferred to Immun-Blot PVDF membranes (YA1701, Solarbio). The membranes were blocked for 2 h in PBS with 0.1% Tween20 (PBST) containing 5% milk and probed overnight with primary antibodies at 4°C. The following primary antibodies were used: Rabbit anti-GAPDH antibody (1:3,000 dilution, Bioss, Beijing, China); Rabbit anti-ROCK1 antibody (1:500, Bioss); Rabbit anti-cofilin antibody (1:1,000, Abcam, United Kingdom); Rabbit anti-phospho-cofilin antibody (1:1,000, Bioss); Rabbit anti-Arp 2/3 complex antibody (1:500, Bioss). After washing 3 times with PBST, the membranes were incubated for 1 h with a Goat anti-rabbit immunoglobulin (IgG ) secondary antibody (1:3,000, Bioss). Proteins were detected using the Pierce ECL Plus Western blotting substrate kit (32,132 × 3, ThermoFisher Scientific, Beijing, China).
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