Probes for real time pcr

Vinyl chloride permeation tubes were obtained from Kin-Tek (La Marque, TX). Primers and probes for real-time PCR were purchased from Integrated DNA Technologies (Coralville, IA) and ThermoFisher Scientific (Waltham, MA). Sources of antibodies used for western blotting were: anti-catalase, -SOD2, -HO1, -cleaved caspase 1 p20, -NRF2 (Santa Cruz Biotechnology, Dallas, TX); anti-SOD1 (GeneTex, Irvine, CA); anti-SOD3 (Novus, Centennial, CO). Sources of antibodies for the flow cytometry include: FITC-anti-Sca-1 (Ly-6A/E), APC-anti-Flk1 (CD309), APC-eFluor780-anti-CD41, PE-Cyanine7-anti-Sca-1, FITC-anti-Nk1.1, PE-anti-Ly6C, PerCPe710-anti-CD8, PECy7-anti-CD62, APC-anti-CD19, Alexa700-anti-Gr-1, APCe780-anti-CD3, eVolve605-CD11b, and e650-anti-CD4 antibodies were purchased from eBioscience (San Diego, CA). Lipopolysaccharides from Escherichia coli O111:B4 (LPS) were obtained from Sigma-Aldrich Cat# 2630. Fc Block (CD32/CD16) (Leinco Technologies; St. Louis, MO), counting beads (Spherotech; Lake Forest, IL), BSA (Rockland Immunochemicals, Limerick, PA). All other chemicals and enzymes were from Sigma Chemical Co. (St. Louis, MO), or Invitrogen (Carlsbad, CA).

Primers and probes for real-time PCR were obtained from Integrated DNA Technologies (Coralville, IA) and ThermoFisher Scientific (Waltham, MA). All other chemicals and enzymes were from Sigma Chemical Co. (St. Louis, MO), or Invitrogen (Carlsbad, CA).