Celltiter glo luminescent cell viability assay kit mts

The cultured cells were grown in 6-well plates until confluent. A scrape was made through the confluent monolayer with a plastic pipette tip of 1 mm diameter. Afterwards, the dishes were washed twice and incubated at 37°C in fresh RPMI containing 10% fetal bovine serum in the presence or absence of 40 μg/mL of IKKα inhibitor. At the bottom side of each dish, two arbitrary places were marked where the width of the wound was measured with an inverted microscope. Cell motility was expressed as the mean +/− SEM of the difference between the measurements at 0, 24, 48 and 72 h after wound.
Cell viability was measured using a CellTiter-Glo Luminescent Cell Viability Assay Kit (MTS) purchased from Promega Corp (Madison, WI) and used according to the manufacturer's protocol. At 24 h post-transfection with 50 ng/mL of EGF or 50 ng/mL of IL-6 in the presence or absence of 1 μg/mL Dox induced, cells were seeded into 96-well plates (2×10³ cells/well), and cell proliferation was recorded every 24 h for 4 days. Measuring the absorbance at 450 nm using a microplate reader assessed the number of viable cells.

Cell viability was measured using a CellTiter-Glo Luminescent Cell Viability Assay kit (MTS) purchased from Promega Corp. (Madison, WI) and used according to the manufacturer's protocol. For flow cytometry analysis of apoptosis, cells were treated as indicated and harvested. Cells (1×10⁶ cells/ml) were resuspended in binding buffer and 0.5 ml of the suspension was transferred to a microfuge tube. After adding 5 μl Annexin V-FITC and 5 μl PI, cells were incubated at room temperature for 15 min in the dark. Apoptosis was analyzed by the Guava EasyCyte HT Sampling Flow Cytometer (Merck Millipore, Billerica, MA).