Hcc2108

Manufactured by Korean Cell Line Bank

Sourced in United States, Cameroon

The HCC2108 is a laboratory equipment item designed for use in cell line research and cultivation. It serves as an incubator for maintaining optimal environmental conditions, such as temperature and CO2 levels, to support the growth and development of cell cultures. The core function of the HCC2108 is to provide a controlled and stable environment for the cultivation of various cell lines.

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Lab products found in correlation

4 protocols using hcc2108

Cell Culture of Human Lung ADC

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Human lung ADC cell lines were obtained from the American Type Culture Collection (H1792) or the Korean Cell Line Bank (H23, H358, H1299, H1703, H1792, H1975, HCC1171, HCC2108, and SK-LU-1). All cells were grown at 37°C with 5% CO₂ in RPMI-1640 or Dulbecco's modified Eagle's medium (HyClone) containing 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin (HyClone). All cells were monitored for Mycoplasma contamination using a PCR-based method for detection. Detected Mycoplasma were eliminated using BM cyclins (Roche).

Kim Y.J., Hong S., Sung M., Park M.J., Jung K., Noh K.W., Oh D.Y., Lee M.S., Oh E., Shin Y.K, & Choi Y.L. (2016). LYN expression predicts the response to dasatinib in a subpopulation of lung adenocarcinoma patients. Oncotarget, 7(50), 82876-82888.

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Murine and Human Lung Cancer Cell Lines

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LLC1 is a cell line derived from the lung tissue of C57BL/6N mice bearing a tumor resulting from implantation of primary LLC; the cell line was procured from the ATCC (Cat. No. CRL-1642, Manassas, VA, United States). HCC-2108 (lung adenocarcinoma) and NCI-H1435 (lung adenocarcinoma, non-small cell lung cancer) were obtained from the Korea Cell Line Bank (Seoul, Korea). Cells were cultured in a humidified incubator at 37°C under 5% CO₂ and 95% air, in DMEM (Cat. No. LM001-05, Welgene Inc., Gyeongsan-si, Korea) or RPMI1640 (Cat. No. LM011-60, Welgene Inc.) supplemented with fetal bovine serum (FBS, 10%), glutamine (2 mM), penicillin (100 U/ml), and streptomycin (100 μg/ml).

Kang M.J., Kim J.E., Park J.W., Choi H.J., Bae S.J., Choi S.I., Hong J.T, & Hwang D.Y. (2021). Effects of Gallotannin-Enriched Extract of Galla Rhois on the Activation of Apoptosis, Cell Cycle Arrest, and Inhibition of Migration Ability in LLC1 Cells and LLC1 Tumors. Pathology and Oncology Research, 27, 588084.

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Culturing NSCLC and Lung Epithelial Cells

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Healthy BEAS-2B human epithelial lung and H1703 human squamous carcinoma cells (American Type Culture Collection, Manassas, VA, USA), and A549, HCC827, HCC2108, HCC95, HCC1588, and HCC1195 NSCLC cells (Korean Cell Line Bank, Seoul, Korea) were cultured as follows. BEAS-2B cells were cultured using the Bronchial Epithelial Cell Growth Medium Bullet Kit (Lonza Group Ltd., Basel, Switzerland). NSCLC cells were maintained in RPMI-1640 (WELGENE Inc., Gyeongsan-si, Korea) supplemented with 10% heat-inactivated fetal bovine serum, glutamine (2 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL).

Chung J.H., Kim T., Kang Y.J., Yoon S.H., Kim Y.S., Lee S.K., Son J.H., Son B, & Kim D.H. (2020). PAK1 as a Potential Therapeutic Target in Male Smokers with EGFR-Mutant Non-Small Cell Lung Cancer. Molecules, 25(23), 5588.

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Lung Cancer Cell Lines Characterization

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The lung cancer cell lines A549, NCI-H358, NCI-H1792, NCI-H23, SW900 and SW1573 were purchased from the American Type Culture Collection. SK-LU-1, Calu-1, Calu-6 and NCI-H460 were provided by Takashi Takahashi (Nagoya University, Japan). RERF-LC-AD2, LU-65 and LU-99 cells were obtained from the Japanese Cell Research Bank (Osaka, Japan). HCC2108 cells were obtained from Korean Cell Line Bank (Seoul, South Korea). The NCI-H1573 and NCI-H2030 cells were provided from Massachusetts General Hospital Cancer Center. Cells were cultured in RPMI1640 (Invitrogen) with 10% FBS. Characteristic of cell lines used in this study were summarized in Supplementary Table S4. Cells were obtained between 2012 and 2016. Experiments using A549, SW900, LU-65 and HCC2108 cells were done within 6 months from the acquisition of these cells from cell banks. All other cell lines were tested and authenticated by short tandem repeat (STR) analysis with GenePrint 10 System (Promega) by the Japanese Cell Research Bank at the time of submission. Cells were regularly screened for Mycoplasma using a MycoAlert Mycoplasma Detection Kit (Lonza). NVP-BGJ398, GDC-0941, ABT-263, selumetinib, afatinib, and trametinib were obtained from Active Biochem. Compounds were dissolved in DMSO to a final concentration of 10 mmol/l and stored at −20°C when not in use.

Kitai H., Ebi H., Tomida S., Floros K.V., Kotani H., Adachi Y., Oizumi S., Nishimura M., Faber A.C, & Yano S. (2016). Epithelial-to-mesenchymal transition defines feedback activation of receptor tyrosine kinase signaling induced by MEK inhibition in KRAS mutant lung cancer. Cancer discovery, 6(7), 754-769.

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