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Cd45ra vioblue

Manufactured by Miltenyi Biotec
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CD45RA-VioBlue is a monoclonal antibody conjugate used for the detection of the CD45RA protein, which is expressed on a subset of T cells. It can be used in flow cytometry applications to identify and quantify these cell populations.

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Lab products found in correlation

Streptavidin pe, by BioLegend (1 mentions) Cd107a pe, by Miltenyi Biotec (1 mentions) Cd3 viogreen, by Miltenyi Biotec (1 mentions) Cd137 pe, by Miltenyi Biotec (1 mentions) Cd45ro fitc, by Miltenyi Biotec (1 mentions) Cd62l fitc, by Miltenyi Biotec (1 mentions) Cd127 fitc, by Miltenyi Biotec (1 mentions) Cd154 vioblue, by Miltenyi Biotec (1 mentions) Anti tnf α pe, by Miltenyi Biotec (1 mentions) Streptavidin allophycocyanin, by BioLegend (1 mentions) Cd8 pe vio770, by Miltenyi Biotec (1 mentions) Cd20 percp, by Miltenyi Biotec (1 mentions) Facsaria, by BD (1 mentions) Diva software, by BD (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Axl pe, by R&D Systems (1 mentions) Easysep human pan dc pre enrichment kit, by STEMCELL (1 mentions) Cd1c percp efluor 710, by Thermo Fisher Scientific (1 mentions) Cd141 bv711, by BD (1 mentions)

2 protocols using cd45ra vioblue

1

Multiparametric Flow Cytometry Analysis

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The following mAbs were used for staining and flow cytometric measurements: CD3-VioGreen (BW264/56), CD4-allophycocyanin-Vio770 (VIT4), CD8-PE-Vio770 (BW135/80), CD14-peridinin chlorophyll protein (PerCP) (TÜK4), CD20-PerCP (LT20), CD27-FITC (M-T271), CD28-FITC (15E8), CD45RA-VioBlue (T6D11), CD45RO-FITC (UCHL1), CD62L-FITC (145/15), CD107a-PE (1D4B), CD127-FITC (MB15-18C9), CD137-allophycocyanin, CD137-PE (4B4-1), CD154-VioBlue (5C8), CD178-PE (NOK-1), CD197 (CCR7)-allophycocyanin, CD197 (CCR7)-PE (150503), CD279-allophycocyanin (PD1.3.1.3), anti-IL-2-PE-Vio770 (N7.48A), anti-IL-4-PE (7A3-3), anti-IL-17-FITC (CZ8-23G1), anti-IFN-γ-FITC, anti-IFN-γ-PE-Vio770 (45-15), anti-TNF-α-PE, and anti-TNF-α-VioBlue (cA2) (all Miltenyi Biotec).
Soluble biotinylated pHLA-A*0201 molecules loaded with WT137 (VLDFAPPGA), WT1126 (RMFPNAPYL), WT1187 (SLGEQQYSV), WT1235 (CMTWNQMNL), or cytomegalovirus (CMV) pp65495 (NLVPMVATV) were produced as described previously.14 (link) Tetramerization was achieved by binding to streptavidin-PE or streptavidin-allophycocyanin (both BioLegend, San Diego, CA). In each case, 2·5 × 106 cells were stained with 1 µg/ml tetramer for 45 min at 4°C.
Data were acquired using a MACSQuant flow cytometer and analysed with MACSQuantify software (both Miltenyi Biotec).
Schmied S., Gostick E., Price D.A., Abken H., Assenmacher M, & Richter A. (2015). Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4+ and CD8+ memory formation. Immunology, 145(4), 558-569.
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2

Profiling Myeloid Cells in HIV-1 Infection

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Peripheral blood mononuclear cells (PBMC) were isolated from HIV-1-infected patients (approved by Comité de protection des personnes CPP, ID-RCB 2017-A02820-53) or from controls (approved by the Institut National de la Santé et de la Recherche Médicale ethics committee) with Ficoll-Paque PLUS (GE). Informed consent was obtained from all donors, and samples were de-identified prior to use in the study. Total blood DCs were enriched with EasySep human pan-DC pre-enrichment kit (Stemcell Technologies 19251). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780, CD1c PerCPeFluor710 (eBioscience), CD123 Viogreen, CD45RA Vioblue, CD169 (Siglec-1) PE-Vio770 (Miltenyi), Axl PE (Clone #108724, R&D Systems), CD33 PE-CF594, CD141 BV711 (BD) and with a cocktail of antibodies against lineage markers CD19 (Miltenyi), CD3, CD14, CD16 and CD34 (BD) in the FITC channel. Data was acquired on a on a FACS Aria using Diva software (BD) and analysed in FlowJo v10 and GraphPad prism v7 for Mac.
Brouiller F., Nadalin F., Bonté P.E., Ait-Mohamed O., Delaugerre C., Lelièvre J.D., Ginhoux F., Ruffin N, & Benaroch P. (2023). Single-cell RNA-seq analysis reveals dual sensing of HIV-1 in blood Axl+ dendritic cells. iScience, 26(2), 106019.
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