Human hepatocytes (HL-7702) were obtained from the Japanese Collection of Research Bioresources and cultured in RPMI-1640 medium (Thermo, USA) containing 10% fetal bovine serum (FBS) (Hyclone, USA) and 1% penicillin (Thermo, Waltham, MA, USA) humidified with 5% CO2 air at 37 °C. The cells were transfected with Ad-CFTR when they were 80% confluent. The control cells were transfected with Ad-GFP. After 48 h, the transfection efficiency was detected by fluorescence microscope, and then the cells were treated with L-Hcy (100 μmol/L) (Sigma, USA) for another 24 h for further experiments.
Hl 7702
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in United States, Japan
HL-7702 is a human liver cell line used for in vitro research. It is a well-differentiated, non-tumorigenic cell line derived from normal human liver tissue. The cell line is designed for use in cell biology, toxicology, and hepatology research.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
L hcy, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hepg2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hep3b, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Sk hep 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hek293t, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
3 protocols using hl 7702
1
CFTR Transfection in Human Hepatocytes
2
Cell Line Culture Protocols
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Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco BRL, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Gibco) and incubated at 37°C in a humidified chamber containing 5% CO2.
3
Homocysteine Exposure in Hepatocytes
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Hepatocyte cell line HL‐7702 was obtained from the Japanese Collection of Research Bioresources. The cells were cultured in 1640 plus 10% serum and 1% antibiotics. When the cells were 70% confluent, serum was deprived for 24 h to synchronize cells, cells were then cultured with 5% fetal calf serum before treatment with 0, 50, 100, 200, and 500 μM Hcy (Sigma‐Aldrich). To compensate for the short half‐life of Hcy, the compound was replenished every 12 h.
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