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Horseradish peroxidase conjugated goat anti mouse sc 2005

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Horseradish Peroxidase conjugated goat anti-mouse (sc-2005) is a secondary antibody that binds to mouse primary antibodies. It is used in various immunochemical techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and visualize the presence of mouse antigens.

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Lab products found in correlation

Ab33911, by Abcam (2 mentions) Nitrocellulose membrane, by LI COR (2 mentions) Protein assay dye, by Bio-Rad (2 mentions) Mouse anti α tubulin t5168, by Merck Group (2 mentions) Anti rabbit irdye 800, by LI COR (2 mentions) Rabbit anti gapdh sc 25778, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg, by Santa Cruz Biotechnology (2 mentions) Rabbit anti gldc hpa002318, by Merck Group (2 mentions) Rabbit anti myc sc 764, by Santa Cruz Biotechnology (2 mentions) Odyssey system, by LI COR (2 mentions) Mouse anti mycn clone op13, by Merck Group (2 mentions)

Spelling variants of this product

Horseradish peroxidase (230 citations) Sc-2005 (81 citations) Goat anti-mouse (sc-2005) (22 citations) Anti-mouse (sc-2005, (13 citations) Mouse anti-goat (5 citations) Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-2005), (4 citations) Peroxidase-conjugated goat anti-mouse (sc-2005, (2 citations) Horseradish peroxidase-conjugated goat anti-mouse secondary antibody (sc-2005; (2 citations) Horseradish peroxidase–conjugated goat anti–rabbit or anti–mouse IgG (sc-2004 or sc-2005, (2 citations)

4 protocols using horseradish peroxidase conjugated goat anti mouse sc 2005

1

Western Blot Analysis of Cell Lysates

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Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
Alptekin A., Ye B., Yu Y., Poole C.J., van Riggelen J., Zha Y, & Ding H.F. (2019). Glycine decarboxylase is a transcriptional target of MYCN required for neuroblastoma cell proliferation and tumorigenicity. Oncogene, 38(50), 7504-7520.
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2

Immunostaining with Fluorescent Antibodies

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Anti-mouse IL-9 polyclonal antibody (504802) and fluorescein isothiocyanate (FITC)-conjugated goat anti-hamster (Armenian) IgG (405502) were purchased from Biolegend (San Diego, CA, USA). FITC-conjugated anti-major histocompatibility complex (MHC) class 1 (ab25056) was procured from Abcam (Cambridge, MA, USA). Horseradish peroxidase-conjugated goat anti-mouse (sc-2005) and goat anti-rabbit (sc-1004) IgG antibodies were obtained from Santa Cruz Biotechnology, Inc. FITC-conjugated CD8 (553031) and phycoerythrin-conjugated CD4 (553730) antibodies were supplied by BD Biosciences (San Jose, CA, USA).
Do Thi V.A., Park S.M., Lee H, & Kim Y.S. (2018). Ectopically Expressed Membrane-bound Form of IL-9 Exerts Immune-stimulatory Effect on CT26 Colon Carcinoma Cells. Immune Network, 18(1), e12.
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3

Flag and Actin Protein Detection

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Laemmli sample buffer was added to luciferase assay protein extracts and the samples were boiled, centrifuged, and run in a SDS-polyacrylamide gel and the proteins were transferred to a PVDF membrane (EMB Millipore, Darmstadt, Germany). Immunoblot analysis was performed using rabbit polyclonal antibody for Flag (G400) (Cat# F1804; Sigma-Aldrich) and goat polyclonal antibody for Actin (1-19) (Cat# sc-1616; Santa Cruz Technology, Dallas, Texas, USA) as well as Santa Cruz Technology secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (sc-2005) and goat anti-rabbit (Cat# sc-2020).
Figueroa-Romero C., Hur J., Lunn J.S., Paez-Colasante X., Bender D.E., Yung R., Sakowski S.A, & Feldman E.L. (2015). Expression of MicroRNAs in Human Post-mortem Amyotrophic Lateral Sclerosis Spinal Cords Provides Insight into Disease Mechanisms. Molecular and cellular neurosciences, 71, 34-45.
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4

Western Blot Analysis of Cell Lysates

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Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
Alptekin A., Ye B., Yu Y., Poole C.J., van Riggelen J., Zha Y, & Ding H.F. (2019). Glycine decarboxylase is a transcriptional target of MYCN required for neuroblastoma cell proliferation and tumorigenicity. Oncogene, 38(50), 7504-7520.
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