Model 1260 hip als autosampler

Drug concentration was analyzed using Agilent 1260 Infinity HPLC system equipped with Model 1260 quaternary pump, Model 1260 Hip ALS autosampler, Model 1290 thermostat, Model 1260 TCC control module, and Model 1260 diode array detector. The output signal was monitored and processed using Agilent ChemStation software. Chromatographic separation was carried out on a 50-mm × 4.6-mm EC-C18 Agilent Poroshell 120 analytical column with 2.7-µm spherical particles, maintained at 40 °C.
For samples in MeOH: DMSO solution, the optimized mobile phase consisted of a gradient elution of methanol: water (pH 3.5 adjusted with 0.1% formic acid) (0 minute (min), 30:70 v/v; 4 min, 30:70; 4.5 min, 90:10; 7 min, 90:10; 7.5 min, 30:70) at flow rate of 1.1 mL/min over a 12-min run time. The injection volume was 20 µL, and the UV detection wavelength of 360 nm was selected.
For samples in simulated gastric fluid (SGF, which consists of 0.2% w/v sodium chloride, 0.7% w/v hydrochloric acid, deionized water, pH 1.2), the optimized mobile phase consisted of a gradient elution of methanol: water (pH 3.5 adjusted with 0.1% formic acid) (0 min, 30:70 v/v; 3.5 min, 70:30; 6.5 min, 70:30; 7.5 min, 30:70) at flow rate of 1.0 mL/min over a 10-min run time. The injection volume was 50 µL, and the UV detection wavelength of 360 nm was selected.