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Cgp54626 dy647

Manufactured by PerkinElmer
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Sourced in France

CGP54626-DY647 is a fluorescent dye-labeled compound. It is designed for use as a research tool in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Infinite f500 reader, by Tecan (2 mentions) Tag lite buffer, by Tecan (2 mentions) Dy647, by Tecan (2 mentions) Tag lite buffer, by PerkinElmer (2 mentions)

2 protocols using cgp54626 dy647

1

GABA Binding Assay in HEK293 Cells

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HEK293 cells were co-transfected with pRK5 plasmids encoding wildtype or mutant GB1 and GB2 in 96 well plates. Intact cells were washed once with Tag-lite buffer (Cisbio, France) 48 h later and incubated with both 5 nM CGP54626-DY647 (Cisbio, France) and increasing concentrations of GABA for 3 h at 4 °C in Tag-lite buffer. After three washes with Tag-lite buffer, fluorescence was measured as the specific DY647 emission spectrum (665 nm) with an Infinite F500 reader (Tecan, Switzerland).
Shaye H., Ishchenko A., Lam J.H., Han G.W., Xue L., Rondard P., Pin J.P., Katritch V., Gati C, & Cherezov V. (2020). Structural basis of the activation of a metabotropic GABA receptor. Nature, 584(7820), 298-303.
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2

GABA Binding Assay in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were co-transfected with pRK5 plasmids encoding wildtype or mutant GB1 and GB2 in 96 well plates. Intact cells were washed once with Tag-lite buffer (Cisbio, France) 48 h later and incubated with both 5 nM CGP54626-DY647 (Cisbio, France) and increasing concentrations of GABA for 3 h at 4 °C in Tag-lite buffer. After three washes with Tag-lite buffer, fluorescence was measured as the specific DY647 emission spectrum (665 nm) with an Infinite F500 reader (Tecan, Switzerland).
Shaye H., Ishchenko A., Lam J.H., Han G.W., Xue L., Rondard P., Pin J.P., Katritch V., Gati C, & Cherezov V. (2020). Structural basis of the activation of a metabotropic GABA receptor. Nature, 584(7820), 298-303.
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