α lamin b1

Manufactured by Abcam

Sourced in United Kingdom

α-Lamin B1 is a nuclear lamina protein that is a component of the nuclear envelope. It plays a role in the structural organization of the nucleus and the regulation of gene expression.

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Lab products found in correlation

α tubulin, by Merck Group (3 mentions) α fibrillarin, by Thermo Fisher Scientific (2 mentions) α wnv capsid, by GeneTex (2 mentions) α wnv capsid, by Abcam (2 mentions) Alexa fluor fluorescent secondary antibody, by Thermo Fisher Scientific (2 mentions) Ab184224, by Abcam (2 mentions) α strep tag, by Abcam (2 mentions) α wnv capsid, by Merck Group (2 mentions) α denv capsid, by GeneTex (2 mentions) α zikv capsid, by GeneTex (2 mentions) α fibrillarin, by Cell Signaling Technology (2 mentions) α ddx55, by Fortis Life Sciences (2 mentions) α pym1, by Novus Biologicals (2 mentions) α magoh, by Santa Cruz Biotechnology (2 mentions) α rbm8a, by Merck Group (2 mentions) α aif, by Cell Signaling Technology (2 mentions) Hrp conjugated secondary antibodies α mouse or α rabbit, by Cytiva (2 mentions) α flag tag, by Merck Group (2 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

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Lamin B1 (174 citations)

5 protocols using α lamin b1

Antibody Panel for Flavivirus Detection

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The following antibodies were used: α-strep tag (ab184224, abcam), α-FLAG tag (F7425, Sigma), α-V5 (A190–120A, Bethyl), α-tubulin (T6199, Sigma), α-WNV capsid (abcam, ab21673), α-WNV capsid (Genetex, GTX131947 ), α-WNV capsid (Sigma, SAB3500912), α-DENV capsid (Genetex, GTX103343), α-ZIKV capsid (Genetex, GTX133317), α-fibrillarin (Thermo Fisher Scientific, AMSA6771), α-fibrillarin (Cell Signaling Technologies, 2639S), α-DDX55 (Bethyl, A303–027A), α-PYM1 (Novus, NBP2–46366), α-MAGOH (Santa Cruz, sc-56724), α-RBM8A (Sigma, HPA018403), α-AIF (Cell Signaling, 5318S), α-Lamin B1 (Abcam, ab231282). The α-flavivirus glycoprotein (4G2) hybridoma was provided by Dr. M. Diamond (Washington University). Alexa-Fluor fluorescent secondary antibodies were from Life Technologies. HRP-conjugated secondary antibodies (α-mouse or α-rabbit) were from Amersham.

Li M., Johnson J.R., Truong B., Kim G., Weinbren N., Dittmar M., Shah P.S., Von Dollen J., Newton B.W., Jang G.M., Krogan N.J., Cherry S, & Ramage H. (2019). Identification of Antiviral Roles for the Exon-Junction Complex and Nonsense-Mediated Decay in Flaviviral Infection. Nature microbiology, 4(6), 985-995.

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Antibody Panel for Flavivirus Detection

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Comprehensive Antibody Catalog for Molecular Research

Cited 2 times

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Primary antibodies used in these experiments were α-Tubulin (Sigma-Aldrich, T5168; Abcam, ab6046 and ab4074), α-A3B (5210-87-13, custom^{68 (link)}), α-Flag (Sigma-Aldrich, F1804), α-Topoisomerase I (Abcam, ab109374), α-Lamin B1 (Abcam, ab16048), α-IgG2a (Sigma-Aldrich, M5409), α-HA (Cell Signaling Technology, 3724S), α-GFP (Abcam, ab290, Lot GR3251545 and GR3270983 for ChIP), α-mCherry (Abcam, ab167453) α-HNRNPUL1 (gift from S. Wilson, University of Sheffield, UK), α-rabbit IgG Isotype Control (Invitrogen, 02-6102, lot RI238244), α-RNA/DNA Hybrid S9.6 (Kerafast, ENH001 or obtained in house from a hybridoma cell line^{69 (link),70 (link)}), α-dsDNA (Abcam, ab27156) and α-gamma-H2AX (Novus, NB100-384). Secondary antibodies used were α-rabbit IRdye 800CW (LI-COR, 827-08365), α-mouse IRdye 680LT (LI-COR, 925-68020), α-rabbit HRP (Cell Signaling Technology, 7074P2 or Sigma-Aldrich, A0545) and α-mouse HRP (Cell Signaling Technology, 7076P2 or Sigma-Aldrich, A8924), Alexa Fluor 488 goat anti-mouse IgG (Invitrogen, A-11029), Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, A-11032), Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen, A-11034), Alexa Fluor 647 goat anti-mouse IgG (Invitrogen, A-21236) and Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen, A-11037).

McCann J.L., Cristini A., Law E.K., Lee S.Y., Tellier M., Carpenter M.A., Beghè C., Kim J.J., Sanchez A., Jarvis M.C., Stefanovska B., Temiz N.A., Bergstrom E.N., Salamango D.J., Brown M.R., Murphy S., Alexandrov L.B., Miller K.M., Gromak N, & Harris R.S. (2023). APOBEC3B regulates R-loops and promotes transcription-associated mutagenesis in cancer. Nature Genetics, 55(10), 1721-1734.

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Western Blot Analysis of Histone Modifications

Cited 1 time

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Protein extracts obtained as previously indicated (T, Ns, Ni or C), were separated by sodium dodecyl sulfate polyacrylamide gel, electrophoresed by (SDS-PAGE). Briefly, 40 μg of each extract was loaded in a 12% SDS-PAGE and transferred to 0.45 μm nitrocellulose membranes (BIO-RAD). For western blot (WB) assays, membranes were stained with Ponceau S Red staining solution (Sigma-Aldrich) and incubated 1 h with 5% non-fat milk. Then were incubated overnight (ON) with α-TRF-like I (1:2,500), α-TRF-like III (1:1,000), α-EhKMT4 (1:1,000) (Borbolla-Vázquez et al., 2015 (link)); α-H3K27me3 (1:1,000, Cell Signaling Technology), α-H4K20me3 (1:1,000, Abcam), α-Lamin B1 (1:1,000, Abcam), α-actin (1:1,000, Sigma-Aldrich) or α-Myc (1:1,000, Cell Signaling Technology) antibodies and then, incubated for 2 h with the corresponding horseradish peroxidase (HRP) labeled secondary antibodies (1:8,000, Santa Cruz Biotechnology). Protein bands were detected and visualized by chemiluminescence on X-ray films. All experiments were repeated at least three times.

Rendón-Gandarilla F.J., Álvarez-Hernández V., Castañeda-Ortiz E.J., Cárdenas-Hernández H., Cárdenas-Guerra R.E., Valdés J., Betanzos A., Chávez-Munguía B., Lagunes-Guillen A., Orozco E., López-Canovas L, & Azuara-Liceaga E. (2018). Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research. Frontiers in Cellular and Infection Microbiology, 8, 341.

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Auxin-Induced H3K9me2/3 Dynamics

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HP1α-AID-sfGFP cells were treated with control media or 1 mM Auxin for 16 hr, then immunostained with α-H3K9me2/3 (Cell Signaling, mouse mAb #5327) and α-Lamin B1 (Abcam ab16048), as described above, and fixed-cell images obtained as above. Lamin B1 stain was used in FIJI to define a nuclear periphery mask, and enrichment was calculated as average intensity within the periphery mask divided by average intensity of the nuclear interior.

Strom A.R., Biggs R.J., Banigan E.J., Wang X., Chiu K., Herman C., Collado J., Yue F., Ritland Politz J.C., Tait L.J., Scalzo D., Telling A., Groudine M., Brangwynne C.P., Marko J.F, & Stephens A.D. (2021). HP1α is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics. eLife, 10, e63972.

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