Aviii hd 600

Manufactured by Bruker

The AVIII HD 600 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced research and analysis. It features a 600 MHz superconducting magnet and delivers exceptional resolution and sensitivity for a wide range of applications. The AVIII HD 600 is a versatile instrument that can be configured with various accessories and software to meet the specific needs of researchers and analytical laboratories.

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Lab products found in correlation

Biacore 3000, by GE Healthcare (1 mentions) Iblot 2 gel transfer device, by Thermo Fisher Scientific (1 mentions) Biacore 8k, by GE Healthcare (1 mentions) Envision 2103, by PerkinElmer (1 mentions) Spectramax i3x, by Molecular Devices (1 mentions) Dpx 400, by Bruker (1 mentions) Lh 20, by GE Healthcare (1 mentions) Lc 6ad instrument, by Shimadzu (1 mentions) 7890a instrument, by Agilent Technologies (1 mentions) 1100 series lc msd ion trap mass spectrometer, by Agilent Technologies (1 mentions) Ods a column, by Shimadzu (1 mentions) V 650 spectrophotometer, by Jasco (1 mentions) Chiralpak ad h column, by Daicel (1 mentions) Av 400, by Bruker (1 mentions) Avii 500, by Bruker (1 mentions) Avance 3 300, by Bruker (1 mentions)

5 protocols using aviii hd 600

NMR Characterization of Isolated Bioactive Molecule

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The small molecule isolated from the purified MRJP1 oligomer was further confirmed by NMR analysis. NMR spectra in CDCl₃ were recorded on a Bruker AV III HD-600 instrument for ¹H and ¹³C using standard pulse programs and acquisition parameters. Chemical shifts were reported in δ (ppm) referencing to the NMR solvent used. The data were consistent with the references^{37 (link)} and^{69 (link)}.
¹H NMR (600 MHz, CDCl₃) δ: 5.36 (d, J = 4.8 Hz, 1H, 6-H), 4.71 (s, 1H, 28-H), 4.66 (d, J = 1.2 Hz, lH, 28-H), 3.55–3.50 (m, lH, 3α-H), 2.31–2.28 (m, 1H), 2.25–2.21 (m, 2H), 2.12–2.07 (m, 1H), 2.03–1.85 (m,2H), 1.61–1.52 (m, 4H), 1.51–1.49 (m, 2H), 1.45–1.40 (m, 3H), 1.31–1.25 (m, 2H), 1.25 (s, 2H), 1.19–1.12 (m, 3H), 1.11–1.05 (m, 2H), 1.03 (d, J = 6.6 Hz, 3H, 26-H₃), 1.02 (d, J = 7.2 Hz, 3H, 27-H₃), 1.01 (s, 3H, 19-H₃), 0.99–0.97 (m, 1H), 0.95 (d, J = 6.6 Hz, 3H, 21-H₃), 0.93–0.87 (m, 1H), 0.68 (s, 3H, 18-H₃).
¹³C NMR (150 MHz, CDCl₃) δ: 156.9 (24-C), 140.8 (5-C), 121.7 (6-C), 106.0 (28-C), 71.8 (3-C), 56.8 (14-C), 56.1 (17-C), 50.2 (9-C), 42.4 (4-C), 42.4 (13-C), 39.8 (12-C), 37.3 (1-C), 36.5 (10-C), 35.8 (20-C), 34.8 (23-C), 33, 9 (25-C), 32.0 (7-C), 31.9 (8-C), 31.7 (2-C), 31.0 (22-C), 28.2 (16-C), 24.3 (15-C), 22.0 (27-C), 21.9 (26-C), 21.1 (11-C), 19.4 (19-C), 18.7 (21-C), 11.9 (18-C).

Tian W., Li M., Guo H., Peng W., Xue X., Hu Y., Liu Y., Zhao Y., Fang X., Wang K., Li X., Tong Y., Conlon M.A., Wu W., Ren F, & Chen Z. (2018). Architecture of the native major royal jelly protein 1 oligomer. Nature Communications, 9, 3373.

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Multi-Instrument Analytical Techniques

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iBlot™ 2 gel transfer device (Thermo Fisher, IB21001). SpectraMax i3x (Molecular Devices). Biacore 3000 instrument (GE Healthcare). Biacore 8 k (GE Healthcare). 2103 EnVision (PerkinElmer). Orbitrap mass spectrometer (Exactive, Thermo Fisher). CFP430 excitation filter (PerkinElmer, #2100–5250, #113). CFP483 emission filter (PerkinElmer, #2100–5270, #220). General bs50/bs50 dual mirror (PerkinElmer, #2100–4050, #651). Bruker AVIII HD 600. Bruker DPX-400. Agilent ESI-TOF. Octet RED96 (ForteBio). Agilent 6135.

Shirey R.J., Hart J.R., Sridharan B., Novick S.J., Turner L.D., Zhou B., Nielsen A.L., Eubanks L.M., Ueno L., Hixon M.S., Lairson L.L., Spicer T.P., Scampavia L.D., Griffin P.R., Vogt P.K, & Janda K.D. (2021). Synthetic fluorescent MYC probe: Inhibitor binding site elucidation and development of a high-throughput screening assay. Bioorganic & medicinal chemistry, 42, 116246.

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Comprehensive Analytical Techniques for Chemical Analysis

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Optical rotations were measured on a JASCO P2000 automatic digital polarimeter. UV spectra were recorded on a JASCO V-650 spectrophotometer. IR spectra were recorded on a Nicolet 5700 spectrometer using an FT-IR microscope transmission method. NMR spectra were acquired with Bruker AVIIIHD 600, VNS-600, or Mercury-400 spectrometers in DMSO-d₆. HRESIMS spectra were collected on an Agilent 1100 series LC/MSD ion trap mass spectrometer. MPLC system was composed of two C-605 pumps (Büchi), a C-635 UV detector (Büchi), a C-660 fraction collector (Büchi), and an ODS column (450 mm×60 mm, 50 μm, 400 g; YMC). Semi-preparative HPLC was conducted using a Shimadzu LC-6AD instrument with an SPD-20A detector and a Daicel Chiralpak AD-H column (250 mm×10 mm, 5 μm). Preparative HPLC was also performed on a Shimadzu LC-6AD instrument with a YMC-Pack ODS-A column (250 mm×20 mm, 5 μm). Column chromatography (CC) was performed with silica gel (200–300 mesh, Qingdao Haiyang Chemical Inc., Qingdao, China), SF-PRP 512 A (100–200 mesh, Beijing Sunflower and Technology Development Co., Beijing, China), ODS (50 μm, YMC, Japan), and Sephadex LH-20 (GE, Sweden). TLC was carried out on glass precoated silica gel GF254 plates. Spots were visualized under UV light or by spraying with 10% sulfuric acid in EtOH followed by heating. GC analyses were obtained using an Agilent Technologies 7890A instrument.

Zhang C., Zhou J., Yang J., Li C., Ma J., Zhang D, & Zhang D. (2016). Two new phenylpropanoid glycosides from the aerial parts of Lespedeza cuneata. Acta Pharmaceutica Sinica. B, 6(6), 564-567.

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Characterization of Organic Compounds by TLC, NMR, and MS

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Thin layer chromatography (TLC) was performed on aluminium sheets coated with 60 F₂₅₄ silica. All solvents are used anhydrous unless stated otherwise. NMR spectra were recorded on Bruker AV400 (400 MHz), Bruker AVII 500 (500 MHz) or AVIIIHD 600 (600 MHz) instruments in the deuterated solvent stated. All chemical shifts (δ) are quoted in ppm and coupling constants (J), which are not averaged, in Hz. Residual signals from the solvents were used as an internal reference using the stated deuterated solvent. Infrared spectra were recorded on a Perkin-Elmer 1750 IR Fourier Transform spectrophotometer using thin films on a diamond ATR surface (thin film). Only the characteristic peaks are quoted. Melting points were determined using a Stanford Research Systems EZ-Melt. Low resolution mass spectra (m/z) were recorded on an Agilent 6120 spectrometer and high resolution mass spectra (HRMS m/z) on a Bruker microTOF mass analyzer using electrospray ionization (ESI). Compounds were synthesised from commercially available starting materials, and fully characterised by Infrared (IR) Spectroscopy, Mass Spectrometry (ESI-MS, HRMS-ESI) and Nuclear Magnetic Resonance (¹H and ¹³C NMR). Spectra supporting the synthesis of these compounds are provided in the S1 File.

Partridge F.A., Forman R., Willis N.J., Bataille C.J., Murphy E.A., Brown A.E., Heyer-Chauhan N., Marinič B., Sowood D.J., Wynne G.M., Else K.J., Russell A.J, & Sattelle D.B. (2018). 2,4-Diaminothieno[3,2-d]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipworm. PLoS Neglected Tropical Diseases, 12(7), e0006487.

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Isolation and Purification of Compounds 1 and 2

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The relevant E. coli or yeast strains (10 L) were fermented and extracted as described above. The organic extracts were separated and dried under vacuum, then re-dissolved in 20 mL of hexanes and passed over columns of silica gel. After loading, the columns were eluted with a hexanes/acetone gradient solvent system (30:1→5:1, v:v), with analysis of the resulting fractions by GC-MS, to purify 1 and 2. The purified products were dissolved in CDCl₃ or pyridine-d₅ for NMR analysis on a Bruker Avance III-300 or a Bruker AVIII HD 600 instruments, and the solvent signals were used for the standards to determine chemical shifts (the ¹H and ¹³C chemical shifts of CDCl₃ are δ_H 7.26 and δ_C 77.0 ppm; the ¹H and ¹³C chemical shifts of pyridine-d₅ are δ_H 8.70, 7.55 and 7.18, and δ_C 149.6, 135.3 and 123.3 ppm).

Liang J., An T., Zhu J.X., Chen S., Zhu J.H., Peters R.J., Yu R, & Zi J. (2021). Mining of the Catharanthus roseus Genome Leads to Identification of a Biosynthetic Gene Cluster for Fungicidal Sesquiterpenes. Journal of natural products, 84(10), 2709-2716.

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