Sensory testing was performed before CCI or carrageenan injection, and after development of the pain phenotype (10 days after CCI for mice, 11–15 days after CCI for rats and 3 h after carrageenan injection). Mechanical and thermal reaction thresholds were determined with a plantar aesthesiometer and a modified Hargreaves apparatus (Ugo Basile, Comerio, Italy), respectively, at the indicated time points on the affected hind paw. The contralateral side was used as control. Paw thickness before and after carrageenan treatment was measured using a thickness gauge (Model No. 7313; Mitutoyo Corporation, Kawasaki, Japan). For open field experiments, mice were put in an open field arena (50 cm × 50 cm × 50 cm, gray plastic) for 60 or 120 min and their activity was determined by a video tracking system (VideoMot; TSE Systems, Bad Homberg, Germany). All experiments were performed blinded to the randomized treatment group assignment.
Model no 7313
Manufactured by Mitutoyo
Sourced in Japan
The Model No. 7313 is a digital micrometer from Mitutoyo. It features a digital display and a measurement range of 0 to 25 mm.
Automatically generated - may contain errors
2 protocols using model no 7313
1
Sensory Testing and Behavioral Analysis in Neuropathic Pain Models
2
Comprehensive Behavioral Assay Protocol
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An experimenter who was blinded to the randomized treatment group assignment performed all behavioral tests. Touch perception thresholds were measured using the up–down method for obtaining the 50% threshold using von Frey hairs.10 (link) Behavioral response to heat was measured using the Hargreaves method23 (link) (Plantar Test Apparatus; Ugo Basile, Varese, Italy). Motor coordination behavior was analyzed using the Rota-Rod test (Mouse Rota-Rod; Ugo Basile). Paw thickness was measured using a thickness gauge (Model No. 7313; Mitutoyo Corporation, Kawasaki, Japan). Body weight was recorded before and at the end of the experiments. For determination of general activity and explorative behavior, an open-field experiment was performed. The mice were put in the open-field arena (50 × 50 cm, gray plastic), and their activity was determined by a video tracking system (VideoMot; TSE Systems, Bad Homberg, Germany) for 15 minutes. Total distance and regional preference were determined.
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