Hepatic and intestinal mononuclear cells or splenic cells were isolated and labeled with anti-CD11c-PE mAb, anti-NK1.1-PE mAb, anti-CD44-FITC mAb, anti-T cell receptor (TCR)-β-FITC mAb, anti-TCR-β-Allophycocyanin (APC) mAb, anti-TCR-γδ-APC mAb, anti-IFN-γ-APC-mAb (all from eBioscience, San Diego, California, USA), anti-TNF-α-APC-mAb (Biolegend San Diego, California, USA). Stained cells were assessed using a FC500 System (Beckman Coulter, Brea, California, USA) equipped with Summit 5.1 software. For the cytotoxic assay, target cells were labeled in advance with carboxyfluorescein diacetate succinimidylester (CFSE; Molecular Probes, Eugene, Oregon, USA) as indicated and analyzed using flow cytometry.
Anti ifn γ apc mab
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-IFN-γ-APC-mAb is a monoclonal antibody conjugated with Allophycocyanin (APC) that binds to Interferon-gamma (IFN-γ). It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Carboxyfluorescein diacetate succinimidyl ester cfse, by Thermo Fisher Scientific (1 mentions)
Anti cd11c pe mab, by Thermo Fisher Scientific (1 mentions)
Fc500 system, by Beckman Coulter (1 mentions)
Facscanto, by BD (1 mentions)
Facs caliber, by BD (1 mentions)
Anti cd45.1 pe mab, by Thermo Fisher Scientific (1 mentions)
Cytofix cytoperm plus kit, by BD (1 mentions)
Golgiplug solution, by BD (1 mentions)
2 protocols using anti ifn γ apc mab
1
Multiparametric Immune Cell Analysis
2
Measurement of IFN-γ in P14 T Cells
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To examine IFN-γ production in transferred P14 T cells, cells from the lymph nodes were stimulated with 10−7 M gp33 peptide and 1μl/ml of Golgi-Plug solution (BD Biosciences) for 6 hours. Recovered cells were stained with anti-CD45.1-PE mAb (eBioscience), anti-CD8α-PerCP mAb (eBioscience) and anti-Va2-FITC mAb (BD Biosciences). Surface stained cells were permeabilized and stained with anti IFN-γ-APC mAb (eBioscience) by using the Cytofix/Cytoperm Plus kit (BD Biosciences). Diphtheria toxin was purchased from Sigma. Cells were analyzed by FACS Caliber or FACS Canto (BD Biosciences). Flow cytometry data were analyzed using FlowJo software (Tree Star).
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