Avance 500 ft nmr spectrometer

The optical rotation data were measured in MeOH with a Jasco P-2000 Polarimeter (JASCO Inc., Tokyo, Japan). The infrared spectra were acquired on Shimadzu IR Affinity-1S Fourier Transform Infrared Spectrophotometer. The UV spectra were obtained from LAMBDA 265 UV/Vis Spectrophotometer (PerkinElmer Inc., Waltham, MA, USA). The 1D and 2D-NMR spectra were recorded with a Bruker Avance 500 FT-NMR spectrometer (Bruker Inc., Bremen, Germany). The HR-ESI-MS data were generated at the Mass Spectrometry Laboratory of the Chung Hsing University with a Thermo LTQ Orbitrap XL™ Hybrid Ion Trap-Orbitrap Mass Spectrometer (Thermo Scientific Inc., Waltham, MA, USA). Column chromatography was performed using LiChroCART Si 5 µM gel (Merck, Darmstadt, Germany) and Sephadex LH-20 (GE Healthcare Life Sciences Inc., Marlborough, MA, USA). The TLC (thin-layer chromatography) analysis was carried out using aluminum pre-coated Si plates (Silica Gel 60 F-254; Merck). The spots were visualized using a UV lamp at λ = 254 nm and detected by spraying with 10% H₂SO₄ alcohol solution and heating at 125 °C. Semi-preparative HPLC was performed using a normal phase column (Luna 5μm Silica 100 Å, 250 × 10 mm; Phenomenex Inc.) on a Precision Instruments IOTA 2 Refractive Index Detector system.

¹H and ¹³C NMR spectra were recorded on a Bruker Avance 500 FT-NMR spectrometer. HR-MS (high-resolution mass spectra) were obtained by a Thermo Scientific Q Exactive mass spectrometer, operated in heated electrospray ionization (HESI) mode and coupled with the Thermo Scientific UltiMate 3000 system. UV-Vis absorption spectra were recorded on a Thermo Scientific Evolution 201 UV-Visible Spectrophotometer. Photo-emission, excitation spectra, and lifetimes were recorded on an Edinburg spectrometer FLS-980 equipped with a Xe light source and an MCP-PMT detector in cooled housing (−20 °C), which covers a range of 200–870 nm. Lifetime data were analyzed with the F980 software package. Emission traces were recorded on an FLS-980 using the multiple scan mode and the shutter was set to always open. UV-portable UV lamps were used as light sources in the photoactivation processes. The optical power densities of these UV light sources were measured by a CEL-NP2000 optical power meter (Beijing CeauLight Technology Co., Ltd.). All measurements were performed at room temperature (RT).