The slides of SKOV3 cells were imaged after immunofluorescence staining, which was performed with JMJD6 (Abcam, 1:50) and p53 (Santa Cruz, 1:50) primary antibodies. Alexa Fluor 488-conjugated goat-anti-rabbit antibody (Invitrogen, 1:100) and Alexa Fluor 594-conjugated goat-anti-rat antibody (Invitrogen, 1:100) were used as the secondary antibodies. Then, the cells were stained with 5 μg/ml DAPI for 10 min, followed by washing and imaging using a Leica TCS SP5 confocal microscope. SKOV3 cells stained with secondary antibodies but without primary antibodies were used as the negative controls.
Alexa fluor 594 conjugated goat anti rat antibody
Manufactured by Thermo Fisher Scientific
The Alexa Fluor 594-conjugated goat-anti-rat antibody is a secondary antibody that binds to rat primary antibodies. The Alexa Fluor 594 dye is conjugated to the antibody, allowing for fluorescent detection and visualization of the target protein or molecule in various immunoassay applications.
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Lab products found in correlation
Jmjd6, by Abcam (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
Dm5500b microscope, by Leica (1 mentions)
Vectashield containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions)
Las af software, by Leica (1 mentions)
2 protocols using alexa fluor 594 conjugated goat anti rat antibody
1
Immunofluorescence Staining of SKOV3 Cells
2
Fluorescence Microscopy of HA-tagged Cells
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For fluorescence microscopy, cells were washed with phosphate-buffered saline (PBS), allowed to adhere to coverslips at a density of 2 × 106 cells/ml, and fixed with 1% formaldehyde in PBS. After permeabilization with PBS–0.1% Triton X-100 and blocking with 3% bovine serum albumin in PBS, cells were incubated with monoclonal rat anti-HA 3F10 antibody (Roche) diluted 1:200 in blocking solution. As a secondary antibody, Alexa Fluor 594-conjugated goat anti-rat antibody (Invitrogen) was used after dilution to 1:2,000 in blocking solution. After the final washing steps, the samples were mounted in Vectashield containing 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratories). Images were made with a Leica DFC360FX monochrome charge-coupled-device camera mounted on a Leica dm5500 B microscope and analyzed using LAS AF software (Leica).
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