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Ni 9401

Manufactured by National Instruments
Sourced in United States

The NI-9401 is a digital input/output (DIO) module that provides 8 channels of 5 V/TTL-compatible digital I/O. The module can be used to interface with a variety of digital devices and sensors. It is designed to be used with National Instruments' CompactDAQ and CompactRIO hardware platforms.

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5 protocols using ni 9401

1

Inkjet Apparatus for High-Speed Imaging

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A home-built inkjet apparatus was employed for this experiment. The system comprised a piezoelectric inkjet nozzle with a diameter of 40 μm (MJ-ATP-01-040 DLC, MicroFab Technologies), a waveform driver (JetDrive, MicroFab Technologies), a nano-pulsed flashlight source (NP-1A, SUGAWARA Laboratories Inc), a CCD camera (avA1000-100gm, Basler) with a high magnification zoom lens (Zoom 6000 Lens System, Navitar), and the 5 × objective lenses (Infinity Corrected Long Working Distance Objective, Mitutoyo). The inkjet nozzle belongs to a squeeze-mode design where a piezo transducer wrapped the outside of a glass capillary tube. The inkjet nozzle pushes out the liquid when receiving the trigger pulse at a jetting frequency of 10 Hz. Based on single-flash high-speed imaging, all jetting images were obtained by the ultra-short pulse flashlight and the CCD camera. The drop watcher system employs a stroboscopic principle to capture the jetting image. The strobe trigger delay time can be adjusted in 1 μs increments. The very short flashlight with a duration of 180 ns avoided significant motion blurring. These components were electrically synchronized and controlled by an embedded controller (cRIO-9035 and NI-9401, National Instruments).
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2

Electrical Stimulation System for Cell Culture

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A schematic of the electrical stimulation system is depicted in Fig. S2. Electrical stimuli were delivered to the culture chamber by a 4-channel, current-controlled stimulator (Digitimer D330 MultiStim) that has a maximum output of 500 mA per channel. The stimulator was remotely controlled by a high-speed TTL pulse generator (National Instruments NI-9401, 100 ns update rate), which was connected to the stimulator by customized wiring between a 25-pin and a 15-pin D-sub connector. The timing and duration of stimulus pulses for each of the 4 independent channels were user-specified in a custom LabVIEW program.
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3

Operant Behavior Testing Platform for Neuroscience Research

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The operant behavior testing (OBT) components were obtained from Med Associates, Inc. (Fairfax, VT): nose poke (ENV-114AM), cue light (ENV-221M), tone generator (ENV-223AM), pellet dispenser (ENV-203) and pellet receptacle (ENV-200R2M). Lithium-Ion batteries (12 V, 10 Ah Model: CR12V10Ah with BMS protection circuit) were purchased from Dakota Lithium Battery (Seattle, WA). An MD-ROOC uses one battery at a time. A bidirectional digital I/O interface (NI 9401), wireless data acquisition unit (cDAQ 9191) and interface software (LabVIEW 2016) originated from National Instruments (Austin, TX). The wireless-LAN router (E1200 N300 Wi-Fi Router) was from Linksys (Irvine, CA). The battery delivers the power for all OBT and National Instruments components. A locally constructed digital interface contains an in-house built printed circuit board that accepts digital inputs to control MedAssociates components as well as receiving signals from the nose poke. (Supplementary Information Figures S.13).
Ordinarily, OBT componentsuse 28 V for both power and control signals. However, they can be configured, or modified, to use 12 V signals instead. Table 1 lists the modifications neccessary to allow the OBT components to be operated at 12 V.
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4

Gas-Sensing PID Electrode Setup

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For the set-up of the PID electrode we used a high-voltage power supply PS350 from Stanford Research Systems, Sunnyvale, CA, USA; a transimpedance amplifier IVC102 from Burr-Brown, Tucson, AZ, USA; a conductive O-ring from Parker Chomerics, Woburn, MA, USA; a commercial PID sensor piD-TECH from AMETEK MOCON, Brooklyn Park, MN, USA; a bare MgF2 glass window purchased from AOTK, Xiamen, China; a Krypton gas fill from PKR 106-6, Heraeus Noblelight, Banbury, Great Britain; a National Instruments CompactDAQ data acquisition platform cDAQ-9178 chassis with modules NI 9401 and NI 9201, from National Instruments, Austin, TX, USA, and two independent gas lines from GFC mass flow controller, Aalborg Instruments & Controls, Orangeburg, New York, NY, USA. For the acquisition of data we used LabVIEW LabVIEW 2011, National Instruments, Austin, TX, USA, with a software routine developed by the authors and available at the GitHub (https://github.com/INLnano/LabVIEW).
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5

Dual-Wavelength Fiber Photometry System

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A dual-wavelength fiber-photometry system (Doric Lenses) was assembled with a dichroic mini cube (iFMC4), 405-nm and 465-nm connectorized LEDs, a fluorescence detector/amplifier, then connected with a pyPhotometry controller board (1.0.2) and operated by Python script provided by pyPhotometry. Calcium-dependent fluorescence (465-nm excitation; F465) and isosbestic control fluorescence (405-nm excitation; F405) were monitored at 130-Hz sampling rate using 1-color time-division mode (alterations), and the data were analyzed by a custom-built LabView software. Isosbestic F405 fluorescence was fitted to F465 signals by least mean squares fitting (F405fitted). Motion-corrected fluorescence signal (dF/F) was calculated by: (F465 – F405fitted)/F405fitted. Both breathing monitoring (PowerLab) and fiber photometry systems received a synchronization signal (3.3-V or 5-V TTL) generated by a cDAQ output device (NI-9401, National Instruments) or a Raspberry Pi 4B device.
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