A549, H1975, HCC827, H2030, and UMC-11 cell lines were obtained from Cobioer Biosciences (Nanjing, China). A549 and H2030 harbor mutations in KRAS; H1975 and HCC827 harbor EGFR mutations. Authentication of A549, HCC827, and H1975 cell lines was performed using short tandem repeat (STR) DNA profiling at Cobioer Biosciences in 2017. Authentication of H2030 and UMC-11 cell lines was performed using STR in 2019. All cell lines were maintained in RPMI-1640 (Hyclone, Omaha, NE, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 100 U/mL penicillin/streptomycin (Hyclone, Logan, UT, USA). All cell lines were passaged for less than 2 months after thawing. For the experiments that required IFN-γ treatment, the cell cultures were supplemented with the indicated amount of IFN-γ (100–2000 IU/mL).
H2030
Manufactured by Cobioer Biosciences
Sourced in China
The H2030 is a laboratory instrument designed for conducting various analytical and experimental procedures. This product's core function is to precisely measure and maintain specific environmental conditions, such as temperature and humidity, within a controlled testing environment. The H2030 is a versatile piece of lab equipment suitable for use in a wide range of scientific and research applications.
Automatically generated - may contain errors
Lab products found in correlation
H1975, by Cobioer Biosciences (4 mentions)
Hcc827, by Cobioer Biosciences (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Umc 11, by Cobioer Biosciences (1 mentions)
F12k medium, by Boster Bio (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
H2444, by Cobioer Biosciences (1 mentions)
F 12k medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using h2030
1
Cell Line Authentication and Cultivation for Cancer Research
2
Lung Adenocarcinoma Cell Line Characterization
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The human lung adenocarcinoma A549 (ATCC Cat# CRL-7900), HCC827 (KCLB Cat# 70,827), H1975 (ATCC Cat# CRL-5908), H2030 (ATCC Cat# CRL-5914), H23 (ATCC Cat #CRL-5800), and PC9 (BCRJ Cat #0331) cell lines were obtained from Cobioer Biosciences (Nanjing, China). Short tandem repeat (STR) analyses of the A549, HCC827, and H1975 cell lines were performed in 2017, and STR analyses of the H2030 and PC9 cell lines were performed in 2019. A549 cells were maintained in F12K medium (Boster, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 U/mL penicillin, and 0.1 mg/mL streptomycin (HyClone, Logan, UT, USA). The remaining cell lines were cultured in RPMI 1640 medium (HyClone, Omaha, NE, USA). The LLC cell line (ATCC Cat #CRL-1642) was maintained in DMEM (HyClone) supplemented with 10% FBS. All cell lines were incubated at 37 °C in a humidified incubator with 5% CO2. All cell lines were confirmed to be Mycoplasma-negative (Biothrive Sci. & Tech. Ltd., Shanghai, China), and the cell lines were used within 3 months after resuscitation.
3
Lung Cancer Cell Line Profiling and Maintenance
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The human lung cancer cell lines A549, H2030, HCC827, H1975 and PC9 were purchased from Cobioer Biosciences (Nanjing, China). Authentication of the A549 cell line was performed using short tandem repeat (STR) DNA profiling at Cobioer Biosciences in 2017. Authentication of the H2030 cell line was performed using STR in 2019. The cells were maintained as a monolayer culture in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO, Grand Island, NY, United States) and 1% penicillin/streptomycin (HyClone, Logan, UT, United States).
4
Characterization of Lung Cancer Cell Lines
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Human lung adenocarcinoma A549 (ATCC Cat# CRL-7909), HCC827 (KCLB Cat# 70827), H2444 (ATCC Cat# CRL-5945), H2030 (ATCC Cat# CRL-5914), and H1975 (ATCC Cat# CRL-5908) cell lines were obtained from Cobioer Biosciences (Nanjing, China). Short tandem repeat (STR) analyses were performed as follows: the analyses of the A549, HCC827, and H1975 cell lines were performed in 2017, and the analyses of the H2444 and H2030 cell lines were performed in 2019. A549, H2444, and H2030 cells harbor mutations in KRAS, and H1975 and HCC827 cells harbor EGFR mutations. The A549 cells were cultured in F12K medium containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 100 U/ml penicillin and 0.1 mg/ml streptomycin (HyClone, Logan, NT, USA). The other cell lines were cultured in RPMI 1640 (HyClone) containing 10% fetal bovine serum supplemented with 100 U/ml penicillin and 0.1 mg/ml streptomycin. The cells were maintained at 37°C in a humidified incubator with 5% CO2. All cell lines were confirmed to be Mycoplasma-negative (Biothrive Sci. & Tech. Ltd., Shanghai, China), and the cell lines were used within 3 months after resuscitation.
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