Regorafenib (BAY 73-4506) and dimethyl sulfoxide (DMSO) were purchased from Selleckchem (Houston, TX, USA) and Sigma–Aldrich (St. Louis, MO, USA). Platelet-derived growth factor-beta (PDGF-BB) was purchased from R&D Systems (Minneapolis, MN, USA). Crystal violet and 4′,6′-diamidino-2-phenylindole (DAPI) were from Sigma–Aldrich (Munich, Germany). The following antibodies were used in this study: Phospho-ERK 1/2 (Thr 202/Tyr 204), ERK (Santa Cruz Biotechnology, Dallas, TX, USA), Phospho-SAPK/JNK (Thr183/Tyr185), SAPK/JNK (Cell Signaling, Danvers, MA, USA), von Willebrand factor (vWF, Dako, Hamburg, Germany), alpha smooth muscle actin (α-SMA, Sigma–Aldrich, Munich, Germany) and vinculin (Sigma–Aldrich, MO, USA).
Regorafenib bay 73 4506
Manufactured by Selleck Chemicals
Sourced in United States
Regorafenib (BAY 73-4506) is a multikinase inhibitor compound. It is used as a research tool in pharmaceutical and academic laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Vinculin, by Merck Group (1 mentions)
Von willebrand factor vwf, by Agilent Technologies (1 mentions)
Alpha smooth muscle actin α sma, by Merck Group (1 mentions)
Palbociclib pd 0332991, by Selleck Chemicals (1 mentions)
Cobalt chloride cocl2, by Merck Group (1 mentions)
Fluorouracil 5 fu, by Selleck Chemicals (1 mentions)
Mg132, by Merck Group (1 mentions)
Lactacystin, by Merck Group (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti parp 1, by Santa Cruz Biotechnology (1 mentions)
Anti ptp1b, by Santa Cruz Biotechnology (1 mentions)
Myc tag, by Cell Signaling Technology (1 mentions)
4 protocols using regorafenib bay 73 4506
1
Regorafenib and PDGF-BB Signaling
2
Palbociclib and Regorafenib Treatment
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Palbociclib (PD-0332991) and regorafenib (BAY73-4506) were purchased from Selleckchem (Houston, TX), and dissolved in water and DMSO, respectively. DMSO concentration never exceeded 0.1% (v/v); equal amounts of the solvent were added to control cells. Cobalt chloride (CoCl2) was purchased by Sigma-Aldrich (St. Louis, MO) and dissolved in water.
3
Regorafenib and Fluorouracil Treatment
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Regorafenib (BAY 73–4506, catalog No. S1178) and Fluorouracil (5-FU, catalog No. S1209) were purchased from SelleckChem. The primary and secondary antibodies used for western blotting and immunohistochemical experiments were all purchased from Cell Signaling Technology unless otherwise specified.
4
Colorectal Cancer Cell Line Maintenance
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The colorectal cancer (CRC) cell lines Hct-15, Hct-116, SW-480, DLD1 and HT-29 were maintained in RPMI 1640 medium with 10% fetal bovine serum (FBS); and CCD-18Co, the normal colon fibroblast cell line, was maintained in Eagle’s Minimum Essential medium supplemented with 10% FBS. All cells were incubated at 37 °C in a humidified 5% CO2 atmosphere. Regorafenib (BAY 73-4506) was purchased from Selleck Chemicals and PTP1B inhibitor was purchased from Calbiochem. Lactacystin and MG-132 were obtained from Sigma. For in vitro studies, drugs were dissolved in dimethyl sufoxide (DMSO) at various concentrations and added to cells in RPMI 1640 medium. The final DMSO concentration was 0.1% after adding to the medium. Antibodies for immunoblotting including Caspase-9 and Myc-tag were purchased from Cell Signaling (Danvers, MA); anti- PARP-1 and anti-PTP1B were from Santa Cruz Biotechnology (San Diego, CA); anti-p-Try was obtained from Millipore (Billerica, MA). Others including anti-PITX-1, -RASA1 (RasGAP) and anti-GAPDH were all obtained from Abcam (Cambridge, MA).
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