A middle part of Gast, TA, and EDL muscle samples was fixed in 4% paraformaldehyde overnight and then embedded into paraffin; 4 μm sections were cut and used for H&E staining, following the standard staining procedures. The muscle fibre cross‐sectional diameter was estimated by CellSens image analysis system (Olympus, Tokyo, Japan). Using a microscope (20× objective, Olympus, Tokyo, Japan), a total of 20 views were randomly selected from each slide that contains muscle cut in cross section. The diameter of all the muscle fibre in each view was automatically calculated by the CellSens image analysis system. The muscle fibre diameter of each group was expressed as the percentage of that of the control group.
Cellsens image analysis system
Manufactured by Olympus
Sourced in Japan
The CellSens image analysis system is a software tool designed to capture, process, and analyze microscopic images. It offers a range of image acquisition, processing, and analysis functions to support various applications in life science research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cellsens image analysis system
1
Muscle Fiber Cross-Sectional Analysis
2
Histological Analysis of Gastrocnemius Muscle
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The gastrocnemius muscle was fixed in 10% formalin overnight and then embedded in paraffin. The prepared paraffin block was cut into 4 μm sections for H&E staining to analyze histological changes. The muscle fiber cross-sectional diameter was estimated using the CellSens image analysis system (Olympus, Tokyo, Japan). Using a microscope with a 20× objective, a total of 20 randomly selected views were taken from each slide containing a cross-section of the muscle. The diameter of all muscle fibers in each view was automatically calculated by the CellSens image analysis system. The muscle fiber diameter of each group was expressed as a percentage of the control group [38 (link)].
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