Mouse monoclonal antibodies against cpla2

All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO), Life Technologies (Grand Island, NY), and Thermo Scientific (Rockford, IL), unless otherwise indicated. Recombinant HMGB-1 was purchased from R&D Systems (Minneapolis, MN) and IBL International Corp (Toronto, ON). Rabbit polyclonal antibody against von Willebrand factor, mouse monoclonal antibodies against cPLA2, α-actin and GAPDH were purchased from Santa Cruz (Santa Cruz, CA). Streptozotocin (STZ) was purchased from Sigma. All the other reagents were obtained from standard commercial suppliers unless otherwise noted.

VEGF-A was acquired from Peprotech (Rocky Hill, NJ, USA); PGE2 came from Cayman Chemical (Ann Arbor, MI, USA); mouse monoclonal antibodies against cPLA₂ were purchased from Santa Cruz (Santa Cruz, CA, USA); mouse monoclonal antibody against GAPDH was purchased from Abcam (Cambridge, UK); rabbit polyclonal antibodies against phospho-cPLA₂ and COX-2 were purchased from Cell Signaling Technology (Danvers, MA, USA); secondary goat anti-mouse TEXAS RED conjugated antibody was purchased from Calbiochem; and secondary goat anti-rabbit IRDye 680 conjugated antibody was purchased from LI-COR (Lincoln, Dearborn, MI, USA). Cisplatin and dexamethasone were purchased from Sigma-Aldrich (Munich, Germany). Media, antibiotics and other reagents for cell cultures came from Innoprot (Elexalde Derio, Spain).