Chromogranin A (CgA) Bdc2.5 peptide mimetope 1040-31 was synthesized by Genscript at >98% purity. 50:50 DL Poly(lactide-co-glycolide) (PLGA) was purchased from Lactel. High molecular weight Poly(vinyl alcohol) (PVA) was purchased from Alfa-Aesar. Rapamycin and all-trans retinoic acid were purchased from LC Labs. Sodium Butyrate was purchased from Sigma-Aldrich. Antibodies for flow cytometry, including PE-CD40, APC-Cy7-CD11c, PE-Cy7-CD86, APC-CD80, APC-H7-CD4, V450-CD4, PE-Cy7-CD25, Alexafluor488-FoxP3, A647-ps6 235–6, and PE-IFNγ were purchased from BD Biosciences. PE-LPAM-1 (α4β7) was purchased from Biolegend. eBioscience FoxP3 Fixation/Permeation kit, cell stimulation cocktail (phorbol 12-myristate 13-acetate (PMA) and Ionomycin), and 1000x Brefeldin A solution were purchased from Thermo fisher. Cytofix/Cytoperm Fixation/Permeabilization kit was purchased from BD. Micro-bicinchoninic acid (mBCA) assay and Vybrant DiO cell-labeling solution was purchased from Thermo Fisher.
High molecular weight poly vinyl alcohol pva
Manufactured by Thermo Fisher Scientific
Sourced in United States
High molecular weight poly(vinyl alcohol) (PVA) is a synthetic polymer that is commonly used in various laboratory applications. It possesses a high molecular weight, which contributes to its unique physical and chemical properties. The core function of this product is to serve as a versatile material for various laboratory procedures and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Cd86 pe cy7, by BD (2 mentions)
Cd40 pe, by BD (2 mentions)
Cd80 apc, by BD (2 mentions)
Ionomycin, by Thermo Fisher Scientific (1 mentions)
Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions)
Vybrant dio cell labeling solution, by Thermo Fisher Scientific (1 mentions)
Cd11c apc cy7, by BD (1 mentions)
Cd4 v450, by BD (1 mentions)
Ifn γ pe, by BD (1 mentions)
Cd25 pe cy7, by BD (1 mentions)
Cell stimulation cocktail, by Thermo Fisher Scientific (1 mentions)
Sodium butyrate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Mannitol, by Merck Group (1 mentions)
Trehalose, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Zombie nir fixable viability kit, by BioLegend (1 mentions)
Foxp3 apc, by BD (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
3 protocols using high molecular weight poly vinyl alcohol pva
1
Immunomodulatory Nanoparticles for T-cell Therapy
2
Encapsulation of MOG and Rapa
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50:50 poly(lactide-co-glycolide) (inherent viscosity range: 0.55–0.75 dL/g) was purchased from Durect Corporation (Birmingham, AL, United States). High molecular weight poly(vinyl alcohol) (PVA) was purchased from Alfa Aesar (Tewksbury, MA, United States). Dichloromethane, mannitol, sucrose, and trehalose were purchased from Sigma Aldrich (St. Louis, MO, United States). Dimethyl sulfoxide was purchased from Thermo Fisher Scientific (Waltham, MA, United States). Myelin oligodendrocyte glycoprotein peptide (MOG35–55, MEVGWYRSPFSRVVHLYRNGK) was synthesized by Genscript (Piscataway, NJ, United States) with ≥98% purity, and Rapa was obtained from LC Laboratories (Woburn, MA, United States).
3
Antigen-specific Immunomodulation Using PLGA
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MOG peptide (MOG35-55, MEVGWYRSPFSRVVHLYRNGK) was synthesized by Genscript (Piscataway, NJ, United States) with >98% purity. Rapamycin was purchased from LC Laboratories (Woburn, MA, United States). 50:50 poly(lactic-co-glycolide) (PLGA) was purchased from LACTEL Absorbable Polymers. High molecular weight poly(vinyl alcohol) (PVA) was purchased from Alpha Aesar (Tewksbury, MA, United States). Dichloromethane (DCM) was purchased from Sigma Aldrich (St. Louis, MO, United States). Dimethyl sulfoxide (DMSO), micro-bicinchoninic acid (mBCA) assay, and eBioscience FoxP3 Fixation/Permeation kit were purchased from Thermo Fisher Scientific (Waltham, MA, United States). Antibodies for flow cytometry, including BV605-CD11c, v450-CD25, PE-T-bet, PE-CD40, PE-Cy-7-CD4, PE-Cy-7-CD86, APC-FoxP3, APC-CD80 were purchased from BD Biosciences. Zombie NIR Fixable Viability Kit was purchased from BioLegend.
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