A biological TNF-α assay was performed using the mouse sarcoma cells, WEHI-13VAR (CRL2148; American Type Cell Culture, Manassas, VA), as previously reported [9 (link), 13 (link)]. The TNF-α activity of each sample was calculated by comparing absorbance to that of a standard curve made from dilutions of rat TNF-α (PharMingen, San Diego, CA) between 1.2 pg · ml−1 and 1250 pg · ml−1. The lower limit of detection of this assay was 1.2 pg · ml−1. The assays were performed between September 2004 and December 2004.
Rat tnf α
Manufactured by BD
Sourced in India
Rat TNF-α is a recombinant protein used in research applications. It is a cytokine that plays a role in the inflammatory response.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rat tnf α
1
Mouse Tumor Necrosis Factor-α Assay
2
Cichorium intybus Seed Extract against Diabetes
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The seeds of Cichorium intybus were procured from Hamdard Wakf Laboratories, India and was authenticated by chief scientist, Raw Material Herbarium and Museum, CSIR- NISCAIR ( Ref. no. NISCAIR/RHMD/Consult/2014/2538/117). streptozotocin (STZ) was purchased from Sigma Aldrich, St. Louis, USA. The AST, LDH, SOD and CAT diagnostic kits were purchased from Span diagnostics, Surat, India. Rat TNF-α and IL-1β cytometric assay kits were purchased from BD Biosciences, India. All the other biochemicals and chemicals used in the present study were of analytical grade.
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