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Human icam 1 fc

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Human ICAM-1 Fc is a recombinant protein consisting of the extracellular domain of human Intercellular Adhesion Molecule 1 (ICAM-1) fused to the Fc region of human IgG1. ICAM-1 is a cell surface glycoprotein involved in cell-cell adhesion and cell signaling.

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Lab products found in correlation

Human cxcl12, by R&D Systems (3 mentions) Fibronectin, by Merck Group (2 mentions) Human e selectin fc, by R&D Systems (2 mentions) Fluorescein isothiocyanate fitc goat secondary antibody to mouse, by Merck Group (2 mentions) Sirnas on targetplus smartpool, by Thermo Fisher Scientific (2 mentions) Kim127 mouse monoclonal antibody, by American Type Culture Collection (2 mentions) Ficoll histopaque, by Merck Group (1 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Human tlr1 9 agonist kit, by InvivoGen (1 mentions) Rabbit polyclonal anti actin antibody, by Merck Group (1 mentions) Tyrphostin ag490, by Merck Group (1 mentions) Anti mouse igg af488, by Thermo Fisher Scientific (1 mentions) Rat igg2a isotype control, by Thermo Fisher Scientific (1 mentions) Formyl methionyl leucyl phenylalanine (fmlp), by Merck Group (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Human igm, by Jackson ImmunoResearch (1 mentions) Ibrutinib, by Selleck Chemicals (1 mentions) Goat f ab 2 anti human igm, by Southern Biotech (1 mentions) Anti jak2 d2e12, by Cell Signaling Technology (1 mentions) Goat anti human igm, by Southern Biotech (1 mentions)

Spelling variants of this product

ICAM-1 (116 citations) ICAM-1-Fc (18 citations) Recombinant human ICAM-1-Fc (5 citations) Recombinant human ICAM-1/CD54-Fc-chimera (3 citations) Soluble ICAM-1/VCAM-1 conjugated to human Fc (2 citations)

7 protocols using human icam 1 fc

1

Investigating Leukocyte Integrin Activation

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Human TLR1-9 agonist kit was purchased from InvivoGen (San Diego, CA). DPI, fibronectin, and Ficoll Histopaque (1077 and 1119) were purchased from Sigma-Aldrich (St. Louis, MO). Rac1 inhibitor was purchased from Calbiochem (Darmstadt, Germany). RBC lysis buffer was purchased from eBioscience (San Diego, CA). Human ICAM-1 Fc was purchased from R&D Systems (Minneapolis, MN). Allophycocyanin-conjugated CD18 (MEM-48) antibody and mAb24 antibody recognizing the β2-integrin high-affinity conformation were purchased from Abcam (Cambridge, United Kingdom). Monoclonal Kim127 antibody against β2-integrin intermediate affinity conformation (American Type Culture Collection, Manassas, VA) was previously described (Kuwano et al., 2010 (link)).
Chung K.J., Mitroulis I., Wiessner J.R., Zheng Y.Y., Siegert G., Sperandio M, & Chavakis T. (2014). A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase. Molecular Biology of the Cell, 25(19), 2948-2955.
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2

Antibody-Based Protein Interactions

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Human E-selectin/Fc, human ICAM-1/Fc and human CXCL12 were from R&D Systems (R&D Systems, Minneapolis, MN, USA); fluorescein isothiocyanate (FITC) goat secondary antibody to mouse was from Sigma (Sigma-Aldrich, St. Louis, MO, USA); KIM127 mouse monoclonal antibody was from American Type Culture Collection (ATCC, Rockville, MD, USA); 327C and A mouse monoclonal antibodies were kindly provided by dr. Kristine Kikly (Eli Lilly and Co., Indianapolis, IN, USA); Tyrphostin AG490 and rabbit polyclonal anti-actin antibody were from Sigma; rabbit monoclonal anti-JAK2 (D2E12), was from Cell Signaling Technology (Danvers, MA, USA); siRNAs (ON-TARGETplus SMARTpool) were from Thermo Fisher Scientific (Fremont, CA, USA).
Montresor A., Toffali L., Mirenda M., Rigo A., Vinante F, & Laudanna C. (2015). JAK2 tyrosine kinase mediates integrin activation induced by CXCL12 in B-cell chronic lymphocytic leukemia. Oncotarget, 6(33), 34245-34257.
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3

Neutrophil Phagocytosis Assay Protocol

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Human IgM (Jackson ImmunoResearch), Protein A (Pierce Thermo Scientific), Human ICAM-1 Fc (R&D), Neutrophil inhibitory factor (NIF) (R&D), Fibronectin (Sigma-Aldrich), TNFα (Peprotech), and fMLP (Sigma-Aldrich) were purchased. Serum used as a source of iC3b was obtained from healthy volunteers. Blood was drawn and handled according to protocols for protection of human participants approved by the Brigham and Women’s Hospital Institutional Review Board, and all volunteer participants gave written informed consent. Anti-human CD11b clone ICRF44 (Biolegend; cat#301310), anti-human CD11b clone M1/70 (Biolegend; cat#101216) and rat IgG2a isotype control (eBioscience; cat#14-4321-82), anti-human CD32a clone IV.3 (Stemcell Technologies; cat#60012) and secondary antibodies anti-mouse IgG AF488 (Invitrogen; cat#A28175) and anti-mouse IgG AF594 (Invitrogen; cat#R37121) were purchased as indicated and used at 1:100-1:1000. Anti-endoglin/CD105 (Biolegend; cat#323202) and rabbit anti-mouse (Dako; cat#Z0259) antibody were used at 1:250 and 1:500 respectively.
Saggu G., Okubo K., Chen Y., Vattepu R., Tsuboi N., Rosetti F., Cullere X., Washburn N., Tahir S., Rosado A.M., Holland S.M., Anthony R.M., Sen M., Zhu C, & Mayadas T.N. (2018). Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment. Nature Communications, 9, 5058.
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4

Immune Cell Adhesion Protein Assay

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Human E-selectin/Fc, human ICAM-1/Fc, human VCAM-1/Fc and human CXCL12 were from R&D Systems (R&D Systems, Minneapolis, MN, USA); goat anti-human IgM was from SouthernBiotech (SouthernBiotech, Birmingham, AL, USA); fluorescein isothiocyanate (FITC) goat secondary antibody to mouse was from Sigma (Sigma-Aldrich, St. Louis, MO, USA); KIM127 mouse monoclonal antibody was from American Type Culture Collection (ATCC, Rockville, MD, USA); 327A mouse monoclonal antibody was kindly provided by Dr. Kristine Kikly (Eli Lilly and Co., Indianapolis, IN, USA); goat F(ab’)2 anti-human IgM was from Southern Biotech (Southern Biotech, Birmingham, AL, USA); Tyrphostin and rabbit polyclonal anti-actin antibody were from Sigma; Ibrutinib was from Selleck Chemicals (Selleck Chemicals, Houston, TX, USA); PE mouse anti-BTK (pY223) antibody was from BD Biosciences (BD Biosciences, San Jose, CA, USA); rabbit monoclonal anti-JAK2 (D2E12) was from Cell Signaling Technology (Danvers, MA, USA); siRNAs (ON-TARGETplus SMARTpool) were from Thermo Fisher Scientific (Fremont, CA, USA).
Montresor A., Toffali L., Rigo A., Ferrarini I., Vinante F, & Laudanna C. (2018). CXCR4- and BCR-triggered integrin activation in B-cell chronic lymphocytic leukemia cells depends on JAK2-activated Bruton’s tyrosine kinase. Oncotarget, 9(80), 35123-35140.
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5

Coating Ibidi Microslides for Cell Adhesion

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Channels Ibidi μ-Slide VI0.1 (Ibidi GMBH, Martinsried, Germany) were coated at 4°C with 50 μL of a 10-μg/mL human ICAM-1-Fc or VCAM-1-Fc (R&D Systems, Minneapolis, MN, United States) in phosphate-buffered saline (PBS) (Gibco), rinsed three times with PBS, then blocked with 75 μL of a 2% bovine serum albumin (BSA, Sigma–Aldrich) solution in PBS (Life Technologies) for 25 min, and rinsed again three times with PBS, and finally filled with RPMI before injection of cells.
Robert P., Biarnes-Pelicot M., Garcia-Seyda N., Hatoum P., Touchard D., Brustlein S., Nicolas P., Malissen B., Valignat M.P, & Theodoly O. (2021). Functional Mapping of Adhesiveness on Live Cells Reveals How Guidance Phenotypes Can Emerge From Complex Spatiotemporal Integrin Regulation. Frontiers in Bioengineering and Biotechnology, 9, 625366.
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6

Confined Migration of Human CD8+ T Cells

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Human CD8+ T cells were purified using MagniSort Human CD8+ T-cell Enrichment Kit (eBioscience) and labeled with 5 μM CMTMR (Invitrogen) 1 d before imaging and incubated with 1 μM lenalidomide or DMSO for 24 h, and recorded migrating confined under agarose block. Briefly, home-made glass bottom dishes were coated with 2 μg/ml human ICAM-1 Fc (R&D) at 4 °C overnight and afterwards rinsed with PBS to remove residual, unbound protein. 500 μl of 0.5% agarose (Biozym Gold Agarose) solution containing 100 ng/ml human CXCL12 (R&D), 50 μM ascorbic acid and fully supplemented R10 medium (RPMI 1640, 10% FCS, glutamine, non-essential amino-acids, β-mercaptoethanol) were poured onto the coated dishes to form approximately 3-mm thick layer and allowed to solidify for 1 h at 4 °C. Finally, dishes were equilibrated for 30 min in a humidified incubator at 37 °C, 5% CO2 and cells were injected between the glass and the agarose layer. Cells were recorded at 37 °C on Nikon Eclipse Ti inverted microscope equipped with Hamamatsu EMCCD C9100-02 camera and tracked using Volocity software (PerkinElmer).
Salzer E., Cagdas D., Hons M., Mace E.M., Garncarz W., Petronczki Ö.Y., Platzer R., Pfajfer L., Bilic I., Ban S.A., Willmann K.L., Mukherjee M., Supper V., Hsu H.T., Banerjee P.P., Sinha P., McClanahan F., Zlabinger G.J., Pickl W.F., Gribben J.G., Stockinger H., Bennett K.L., Huppa J.B., Dupré L., Sanal Ö., Jäger U., Sixt M., Tezcan I., Orange J.S, & Boztug K. (2016). RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics. Nature immunology, 17(12), 1352-1360.
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7

ICAM-1 and Non-Adherent Microfluidic Coatings

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Microfluidic channels (Ibidi Slide IV 0.4) were coated with 10µg/mL human ICAM-1-Fc (R&D Systems) overnight at 4°C. Channels were subsequently blocked with 4% bovine serum album (BSA) for at least 15 min at room temperature, and rinsed with PBS and RPMI medium prior to use. Non-adherent substrates were prepared either by incubating the channels with 5% Pluronic F-127 (Sigma-Aldrich) for at least 30 min at room temperature, or by mPEG SVA treatment following the same protocol as described for bacteria micropatterning.
Garcia-Seyda N., Seveau V., Manca F., Biarnes-Pelicot M., Valignat M.P., Bajénoff M, & Theodoly O. (2021). Human neutrophils swim and phagocytise bacteria. Biology of the cell, 113(1).
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