The silica binding studies were performed using silica-coated quartz crystals (QSensor QSX 303 SiO2) purchased from ATA Scientific (Taren Point, NSW, Australia). Pefabloc, lysozyme, and Benzonase nuclease were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Human serum was obtained from Sigma-Aldrich (Castle Hill, NSW, Australia), while the humanized anti-HER2 monoclonal antibody trastuzumab was purchased from Jomar Life Research (Melbourne, VIC, Australia). The human HER2/ErbB2 protein (His-Tag) was purchased from Sino Biological Inc. (Beijing, China). All biological assays were performed at room temperature with standard phosphate-buffered saline (1 × PBS) at pH 7.4 containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, and 2.7 mM KCl (Sigma Aldrich, Castle Hill, NSW, Australia). All other chemicals were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia) unless otherwise stated.
Human her2 erbb2 protein his tag
Manufactured by Sino Biological
Sourced in China
Human HER2/ErbB2 protein (His-Tag) is a recombinant protein produced in insect cells. It contains a His-Tag at the C-terminus. HER2/ErbB2 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases.
Automatically generated - may contain errors
Lab products found in correlation
Benzonase nuclease, by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Human serum, by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Pefabloc, by Merck Group (1 mentions)
Human ab serum, by Harvard Bioscience (1 mentions)
L glutamine, by Merck Group (1 mentions)
Gentamycin, by Merck Group (1 mentions)
Crm197, by Merck Group (1 mentions)
Rpmi 1640, by Harvard Bioscience (1 mentions)
2 protocols using human her2 erbb2 protein his tag
1
Silica Binding Studies of Biomolecules
2
PBMC Stimulation Assay Protocol
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PBMC samples stored in liquid nitrogen were reestablished in culture medium RPMI1640 supplemented with 10% human AB serum (Biochrom) and 2 mmol/L L-glutamine, 50 mmol/L 2-mercaptoethanol, and 0.1 mg/mL gentamycin (all Sigma Aldrich). Cells were plated in 96-well round-bottom plates at 8 Â 10 5 /well and cultured with CRM197 (Merck-Millipore) at 4 mg/well, with human HER2/ErbB2 Protein (His Tag; Sino Biological Inc.) at 4 mg/well, and with superantigen Staphylococcus Enterotoxin B (0.2 mg/well) as positive control and in culture medium only for assessment of cytokine baselines. Cultures (total volume 200 mL) were maintained for 48 hours (37 C, 5% CO 2 , 95% humidity) and then supernatants were harvested, pooled, and stored at À20 C until analyses.
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