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Ab109123

Manufactured by Abcam
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Sourced in United Kingdom

Ab109123 is a primary antibody that targets the Human CD3 Epsilon protein. It is suitable for use in applications such as flow cytometry and immunohistochemistry. The antibody is purified and supplied as a liquid in PBS buffer.

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Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (2 mentions) Hematoxylin, by Maixin Group (1 mentions) Diaminobenzidine dab, by Maixin Group (1 mentions) Rneasy plus mini rna extraction kit, by Qiagen (1 mentions) Ab140682, by Abcam (1 mentions) Streptavidin magnetic beads 88816, by Thermo Fisher Scientific (1 mentions) Rna to ct 1 step kit, by Thermo Fisher Scientific (1 mentions) Brd3 a302 368a, by Fortis Life Sciences (1 mentions) Ab71165, by Abcam (1 mentions) Lactacystin, by Peptide Institute (1 mentions) Mg132, by Fujifilm (1 mentions) Rad23a, by Cell Signaling Technology (1 mentions) Lamin b1 c 20, by Santa Cruz Biotechnology (1 mentions) Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (1 mentions) α tubulin, by Merck Group (1 mentions)

4 protocols using ab109123

1

Immunohistochemical Analysis of PSMD14

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After fixation of tissues with 10% neutral formalin, 4‐μm‐thick paraffin‐embedded tissue sections were prepared. Following deparaffinized, hydrated, and soaked in 3% H2O2 for 20 min at room temperature, the sections were incubated with anti‐PSMD14 (1 : 500, ab109123, Abcam, Cambridge, UK) antibodies at 4 °C overnight. The slides were incubated with biotinylated goat anti‐rabbit antibodies for 1 h, stained with diaminobenzidine (DAB; Maixin Biotechnology, Fuzhou, China), and then counterstained with hematoxylin (Maixin Biotechnology). The IHC sections were assessed by two independent pathologists blinded to the experimental data. The specimen was scored according to the percentage of positive staining cells (0 = negative; 1 = 1–10%; 2 = 11–50%; 3 = 51–90%; 4 = 91–100%) and the intensity of staining (0 = no staining; 1 = slight staining; 2 = moderate staining; 3 = strong staining). Scores for the percentage and intensity of staining were added. The score of 0–3 was considered as a low‐expression group, and the score of 4–7 was considered as a high‐expression group.
Sun T., Liu Z., Bi F, & Yang Q. (2021). Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2. Molecular Oncology, 15(12), 3639-3658.
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2

Proteomic analysis of protein complexes

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Antibodies to the indicated proteins were purchased from the following vendors: BRD4 (ab128874), BRD2 (ab139690), PSMD14 (ab109123) and PSMD1 (ab140682; all Abcam); BRD3 (A302-368A; Bethyl); tubulin (926-42211; LICOR Biosciences); PSMD4 (3846), PSMD2 (25430), biotin (5597) and H2B (12364; Cell Signaling Technology) and PSMA1 (BML-PW8100-0100; ENZO Life Sciences). IRDye-800 anti-rabbit (926-32211) and IRDye-680RD anti-mouse (926-68072) secondary antibodies were purchased from LICOR Biosciences. Bortezomib (179324-69-7) was purchased from Calbiochem. Streptavidin magnetic beads (88816), NeutrAvidin agarose beads (29200), Lipofectamine RNAiMAX transfection reagent (13778075), RNA-to-Ct 1-Step kit (4392653) and TaqMan gene expression assays were purchased from Thermo Fisher. An RNeasy Plus mini RNA extraction kit (74134) was purchased from Qiagen.
Bashore C., Prakash S., Johnson M.C., Conrad R.J., Kekessie I.A., Scales S.J., Ishisoko N., Kleinheinz T., Liu P.S., Popovych N., Wecksler A.T., Zhou L., Tam C., Zilberleyb I., Srinivasan R., Blake R.A., Song A., Staben S.T., Zhang Y., Arnott D., Fairbrother W.J., Foster S.A., Wertz I.E., Ciferri C, & Dueber E.C. (2022). Targeted degradation via direct 26S proteasome recruitment. Nature Chemical Biology, 19(1), 55-63.
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3

Immunohistochemical Analysis of PSMD14 and USP14 in NSCLC

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Human NSCLC tissues and adjacent non-cancerous tissues were cut into 4 µm slices. After baking at 70 °C for 30 minutes, the slices were dewaxed and hydrated using a xylene and alcohol series, respectively. Endogenous peroxidase activity was blocked by incubation with 3% hydrogen peroxide at room temperature for 10 minutes. Antigen recovery was performed by heating with a pressure cooker in citric acid antigen repair solution (pH 6.0). After blocking with 10% goat serum, the slices were incubated with rabbit anti-PSMD14 monoclonal antibody (ab109123) or USP14 polyclonal antibody (ab71165; Abcam, Cambridge, UK) at 4 °C overnight. The tissue sections were then washed in phosphate-buffered saline (PBS) and incubated with a horseradish peroxidase-conjugated secondary antibody (MaixinBiol, Fuzhou, China) for 1 hour at room temperature. Samples were stained with diaminobenzidine and counterstained with hematoxylin according to the MaxVision kit (MaixinBiol) instructions.
Lei J., Liu X., Liu W., Zhang Y, & Liu Z. (2021). The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer. Annals of Translational Medicine, 9(12), 1019.
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4

Site-Directed Mutagenesis of PSMD14

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Site-directed mutagenesis of PSMD14 was performed with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA) and primer sequences described previously56 (link). MLN4924 (Sequoia Research Products, Pangbourne, UK), MG132 (Fujifilm Wako Pure Chemical), and lactacystin (Peptide Institute, Ibaraki, Osaka, Japan) were purchased from the indicated suppliers. Generation of antibodies against XPC69 (link), RAD23B30 (link), and PSMD457 (link) was described previously. The following antibodies were purchased from manufacturers: XPC (NB100-477, Novus Biologicals, Centennial, CO, USA), DDB2 (AF3297, R&D Systems, Minneapolis, MN, USA), CUL4A (14851-1-AP, Proteintech, Rosemont, IL, USA), multi-ubiquitin (FK2; D058-3, Medical & Biological Laboratories, Nagoya, Japan), mKO2 (PM051M, Medical & Biological Laboratories), PSMD14 (ab109123, abcam, Cambridge, UK), RAD23A (24555, Cell Signaling Technology, Danvers, MA, USA), α-tubulin (T5168, Merck), and lamin B1 (C-20, Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Sakai W., Yuasa-Sunagawa M., Kusakabe M., Kishimoto A., Matsui T., Kaneko Y., Akagi J.I., Huyghe N., Ikura M., Ikura T., Hanaoka F., Yokoi M, & Sugasawa K. (2020). Functional impacts of the ubiquitin–proteasome system on DNA damage recognition in global genome nucleotide excision repair. Scientific Reports, 10, 19704.
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